摘要
为鉴定引起茶褐枯病的病原菌,本研究对病原菌进行分离、纯化和培养,通过柯赫氏法则验证菌株的致病性,并观察病原菌的形态特征,依据病原菌rDNA-ITS、ACT、CAL和TUB2基因进行多基因系统发育分析。结果显示:病原菌分生孢子呈淡蓝色,表面光滑,无隔膜,圆柱状,两端钝或向底部变窄,水滴状斑点,大小为(11.7~29.5)μm×(3.9~7.7)μm,平均为(19.4±4.4)μm×(5.4±0.8)μm,分生孢子梗形成于气生菌丝上,透明,具有隔膜;附着胞为棒状等不规则形状,颜色呈棕色到深棕色,单生。基于病原菌形态学鉴定和多基因系统发育分析结果,将病原菌确定为山茶刺盘孢Colletotrichum camelliae。
To identify the pathogen causing tea brown blight disease,we isolated,purified and cultivated the pathogenic fungus from the diseased tea leaves.Its pathogenicity was determined according to Koch’s rule,and the morphological characteristics of the pathogen were observed.Phylogenetic analysis of multi-locus sequences,including rDNA-ITS,actin,calmodulin and tubulin 2,was conducted.The results showed that the conidium of the pathogen was hyaline,smooth-walled,aseptate,cylindrical,with obtuse ends or narrowed towards the base,guttulate,(11.7-29.5)μm×(3.9-7.7)μm,and the average was(19.4±4.4)μm×(5.4±0.8)μm.Conidiophores were directly formed from aerial mycelia,hyaline,septate.Appressoria were clavate,irregularly shaped,brown to dark brown,and solitary.Based on phylogenetic analyses and morphology,the pathogen was identified as Colletotrichum camelliae.
作者
王雪
王勇
尹桥秀
李冬雪
任亚峰
江仕龙
王德炉
宋宝安
陈卓
WANG Xue;WANG Yong;YIN Qiaoxiu;LI Dongxue;REN Yafeng;JIANG Shilong;WANG Delu;SONG Baoan;CHEN Zhuo(Key Laboratory of Green Pesticides and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China;Agricultural College, Guizhou University, Guiyang 550025, China;College of Forestry, Guizhou University, Guiyang 550025, China)
出处
《植物保护》
CAS
CSCD
北大核心
2020年第2期101-106,共6页
Plant Protection
基金
国家科技支撑计划(2014BAD23B03)
国家重点研发计划后补助(黔科合平台人才[2018]5262)
贵州省科技重大专项[黔科合重大专项(2012)6012号]
贵州省科技厅-黔南州人民政府农业科技合作专项计划(2013-01)。
关键词
茶褐枯病
山茶刺盘孢
形态特征
致病性分析
系统发育分析
tea brown blight disease
Colletotrichum camelliae
morphological characteristics
pathogenicity analysis
phylogenetic analysis
