茭白胡麻叶斑病病原菌分离与鉴定

Isolation and identification of the pathogen causing leaf spot of Zizania latifolia

茭白胡麻叶斑病发生普遍且严重,已成为茭白的常见病害。为了明确病原菌,先后从茭白主产区采集胡麻叶斑病病叶,各产区分离纯化5~10株病原菌。通过对菌株DNA的内转录间隔区ITS基因、3-磷酸甘油醛脱氢酶GPDH基因、延伸因子EF-1α基因的序列进行分析。结合形态学观察、致病力测定等方法对茭白胡麻叶斑病病菌进行鉴定。结果表明,茭白胡麻叶斑病病原菌为稻平脐蠕孢Bipolaris oryzae,与水稻胡麻叶斑病病原菌一致。本文的研究成果可为茭白胡麻叶斑病的防治提供一种新的思路。

Zizania latifolia leaf spot disease is a common and serious disease.In order to identify the pathogen,we collected infected leaves from different main production areas of Z.latifolia and selected 5 to 10 strains in each area.Identification experiments were performed based on DNA internal transcribed spacer ITS,glyceraldehyde-3-phosphate dehydrogenase(GDPH)and elongation factor EF-1αgene sequences and morphology.The results showed that the pathogen of leaf spot of Z.latifolia was Bipolaris oryzae,which was the same as the pathogen of rice flax leaf spot.This study provides a new idea for the control of leaf spot of Z.latifolia.