一种高效的镰刀菌接种体制备方法

A high-efficiency method for production of uniform inoculum of Fusarium

本文以玉米茎腐病人工接种所需的禾谷镰刀菌Fusarium graminearum和拟轮枝镰刀菌F.verticillioides为材料,比较了三角瓶、聚乙烯袋和牛皮纸袋内用谷物培养基制备接种体的差别。发现在牛皮纸袋内用孢子悬浮液接种玉米粒培养基可高效制备接种体。与三角瓶、聚乙烯袋相比,在牛皮纸袋中制备接种体表现生长点多、透气性好、菌丝生长快、污染率低、质量高、对存放环境要求不严的优点。这种方法可以为玉米茎腐病等相似土传病害的人工接种和病圃建造等提供高质量的接种体。

This study aimed at investigating the differences in flask,polyethylene bag and kraft bag to produce Fusarium graminearum inoculum and F.verticillioides inoculum on a corn grain medium in order to artificially inoculate maize and cause stalk rot.It was found that inoculum could be efficiently prepared by inoculating corn kernel medium with spore suspension in kraft paper bag.In comparison with flask and polyethylene bag,the inoculum in kraft bags has the advantages of many growing points,good air permeability,fast growth of mycelium,low pollution rate,high quality and lax storage environment requirements.This method can be used in rapid production of uniform inoculum on grain medium for soil-borne plant pathogen causing diseases.