清热散结胶囊三维荧光指纹图谱研究

Investigation of three-dimensional fluorescence fingerprint of Qingre Sanjie Capsule

本文采用荧光光谱法对清热散结胶囊的荧光性质进行了研究,对三维荧光图谱测定的实验条件进行了优化,在最佳实验条件下测定了2个厂家14个批号的清热散结胶囊的三维荧光图谱,利用聚类分析法对清热散结胶囊的三维荧光图谱的一致性进行分析,结果表明2个厂家14个批号的56个清热散结胶囊样本的三维荧光图谱一致性良好。采用平均值法建立了清热散结胶囊的三维荧光指纹图谱。

The fluorescence properties of Qingre Sanjie Capsule were investigated by fluorescence spectrometry in this paper.The experimental conditions for the determination of threedimensional fluorescence spectrum were optimized,and the three-dimensional fluorescence spectrum of Qingre Sanjie Capsules with different batches from different manufacturers were determined under the optimum experimental conditions.The consistency of the three-dimensional fluorescence spectra of Qingre Sanjie Capsules was analyzed by cluster analysis.The results showed that the good consistency of the three-dimensional fluorescence spectra of 56 Qingre Sanjie Capsules with 14 batches from 2 manufacturers was observed.The three-dimensional fluorescence fingerprint of Qingre Sanjie Capsule was established by means of average method.