番茄红素对非酒精性脂肪肝大鼠糖脂代谢及炎症水平的影响

Effects of lycopene on metabolism of glycolipid and inflammation in non-alcoholic fatty liver disease rats

目的探讨番茄红素对非酒精性脂肪肝大鼠糖脂代谢及炎症水平的影响。方法 60只成年雄性SD大鼠,按体重随机分为空白对照组、非酒精性脂肪肝模型组、番茄红素20、60 mg/kg干预组,每组15只。空白对照组喂普通饲料,其余3组以高脂高果糖饲料喂养4周建立非酒精性脂肪性肝病模型。造模成功后,干预组分别给予不同剂量的番茄红素灌胃,8周后处死,称取大鼠体重与肝重,计算肝脏指数;苏木精-伊红染色观察肝脏组织形态学变化;收集血清,检测空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,INS)、计算胰岛素抵抗指数(insulin resistance index,HOMA-IR);测定肝功能指标丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST);检测血清甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)及炎症因子白细胞介素-6(interleukin-6,IL-6)、IL-18、IL-1β水平。结果与空白对照组相比,模型组大鼠肝重和肝脏指数分别升高了27%和24%;肝组织出现脂肪变性;血清ALT、TG、TC、LDL-C、IL-6、IL-1β水平显著上升,HDL-C水平显著下降(P<0.05)。与模型组相比,20、60 mg/kg番茄红素干预组大鼠肝重、肝脏指数、血清ALT、TG、FBG、IL-6、IL-1β水平均明显降低(P<0.05),INS、HOMA-IR指数均呈下降趋势;肝脏组织病变呈不同程度减轻,且以60 mg/kg番茄红素组改善效果更为显著。结论番茄红素可通过改善大鼠糖脂代谢、降低炎症因子水平来改善非酒精性脂肪肝。

OBJECTIVE To investigate the effects of lycopene on metabolism of glycolipid and inflammation in rats with non-alcoholic fatty liver disease. METHODSAccording to body weight, sixty male Sprague-Dawley rats were randomly divided into control group, non-alcoholic fatty liver model group, 20, 60 mg/kg lycopene intervention groups, with 15 rats in each group. The control group was fed normal diet, and the other 3 groups were fed high-fat and high-fructose diet for 4 weeks to establish the model of non-alcoholic fatty liver disease. After modeling, the intervention groups were fed lycopene of different doses by gavage. After 8 weeks of continuous feeding, the rats were sacrificed, the body weight and liver weight were weighed, and the liver index was calculated. Hematoxylin-eosin staining was used to observe the morphological changes of liver tissue. Serum was collected, fasting blood glucose(FBG), fasting insulin(INS), and insulin resistance index(HOMA-IR) were calculated;Liver function indicators alanine aminotransferase(ALT), aspartate aminotransferase(AST) were measured;Serum triglycerides(TG), total cholesterol(TC), high density lipoprotein-cholesterol(HDL-C), low density lipoprotein-cholesterol(LDL-C) and levels of inflammatory cytokines interleukin-6(IL-6), interleukin-18(IL-18) and interleukin-1β(IL-1β) were measured. RESULTS Compared with the control group, the liver weight and liver index in the model group increased by 27% and 24%, respectively;And steatosis occurred in the liver tissue;The levels of serum ALT,TG, TC,LDL-C, IL-6, IL-1β were significantly increased, the level of serum HDL-C was significantly decreased(P<0.05). Compared with the model group, liver weight, liver index, levels of serum ALT, TG, FBG, IL-6 and IL-1β were significantly lower in the 20, 60 mg/kg lycopene intervention groups(P<0.05), the INS and HOMA-IR index showed a downward trend;Liver tissue lesions were reduced to different degrees, and the effect was more significant in the 60 mg/kg lycopene group. CONCLUSION Lycopene can improve non-alcoholic fatty liver disease by regulating glycolipid metabolism and reducing levels of inflammatory cytokines in rats.