婴幼儿呼吸道合胞病毒肺炎外周血单核细胞miR-146a和miR-155的表达及与炎症反应关系

Relationship between the expression of miR-146a and miR-155 in peripheral blood mononuclear cells and inflammation response in infants with respiratory syncytial virus pneumonia

目的探讨婴幼儿呼吸道合胞病毒(RSV)肺炎外周血单核细胞(PBMCs)miR-146a和miR-155的表达及与炎症反应关系。方法选取2017年1月-2019年1月三峡大学第三临床医学院葛洲坝集团中心医院收治的84例RSV肺炎患儿作为研究组,根据病情严重程度分为轻度组和重度组。选取同期体检健康婴幼儿80例作为对照组,采集受试者外周血并分离单核细胞,采用实时荧光定量PCR检测单核细胞中miR-146a和miR-155水平,采用酶联免疫吸附法检测血清肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)水平,Pearson法分析两组受试者miR-146a、miR-155水平与炎症因子水平的相关性。结果重度组患儿PBMCs中miR-146a水平明显低于轻度组与对照组(F=31.907,P<0.05),轻度组与对照组比较差异无统计学意义(P>0.05);重度组患儿PBMCs中miR-155水平显著高于轻度组、对照组(F=143.127,P<0.05),轻度组高于对照组(P<0.05);研究组患儿血清TNF-α、IL-1β、IL-6、IL-8水平均高于对照组(t=36.627、39.350、22.743、10.617,P<0.05)。RSV肺炎患儿miR-146a与TNF-α、IL-1β、IL-6、IL-8表达呈负相关(r=-0.473、-0.502、-0.396、-0.337,P<0.05),miR-155与TNF-α、IL-1β、IL-6、IL-8表达呈正相关(r=0.451、0.394、0.359、0.382,P<0.05)。结论 RSV肺炎患儿PBMCs中miR-146a低表达,miR-155高表达,并与患儿疾病严重程度及炎症反应相关。

Objective To investigate the relationship between inflammation response and the expression of miR-146 a and miR-155 in peripheral blood mononuclear cells(PBMCs) of infants with respiratory syncytial virus(RSV) pneumonia. Methods Totally 84 children with RSV pneumonia admitted in the Third Clinical Medical College of Three Gorges University were enrolled in this study from January 2017 to January 2019,and were divided into mild group and severe group.Meanwhile,80 healthy children who took physical examination were selected as control group.Peripheral blood was collected and monocytes were isolated,real-time fluorescent quantitative PCR was used to test the levels of miR-146 a and miR-155 in monocytes.The levels of serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6) and interleukin-8(IL-8) were detected by ELISA.Pearson method was used to analyze the correlation between the levels of inflammatory factors and the expression levels of miR-146 a and miR-155 in the two groups. Results The level of miR-146 a in PBMCs of severe group was significantly lower than that of mild group and control group(F=31.907,P<0.001),but miR-146 a level was not significantly different between the mild group and the control group(P>0.05).The level of miR-155 in PBMCs of severe group was significantly higher than that of mild group and control group(F=143.127,P<0.001),and it was significantly higher in mild group than control group(P<0.001).The levels of serum TNF-α,IL-1β,IL-6 and IL-8 in children with RSV pneumonia were significantly higher than those in the control group(t=36.627,39.350,22.743,10.617,P<0.001).MiR-146 a level was negatively correlated with the expressions of TNF-α,IL-1β,IL-6 and IL-8 in children with RSV pneumonia(r=-0.473,-0.502,-0.396,-0.337,P<0.05),while miR-155 level was positively related to the expressions of TNF-α,IL-1β,IL-6 and IL-8(r=0.451,0.394,0.359,0.382,P<0.05). Conclusion The expression of miR-146 a in PBMCs of children with RSV pneumonia is low,while the expression of miR-155 is high,both of which are related to the severity of disease and inflammatory reaction in children.