MALAT1在慢性鼻窦炎伴鼻息肉中的表达及对糖皮质激素治疗的反应性

Expression and glucocorticoid regulation of MALAT1 in chronic rhinosinusitis with nasal polyps

目的研究长链非编码RNA肺腺癌转移相关转录本1(metastasis-associated lung adenocarcinoma transcript 1,MALAT1)在不同亚型慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)中的表达,及其对糖皮质激素治疗的反应性。方法利用real-time PCR检测MALAT1在嗜酸性粒细胞型和非嗜酸性粒细胞型CRSwNP鼻息肉组织及健康对照鼻黏膜组织中的表达水平,分析MALAT1表达与Th1、Th2、Th17型细胞因子表达的相关性,并比较同一患者经糖皮质激素治疗前后MALAT1的表达变化。结果MALAT1在两种亚型CRSwNP鼻黏膜中的表达均显著低于健康对照组,并且在非嗜酸性粒细胞型CRSwNP组低于嗜酸性粒细胞型CRSwNP组;干扰素-γ(interferon gamma,IFNG)的表达在嗜酸性粒细胞型CRSwNP和非嗜酸性粒细胞型CRSwNP均显著高于健康对照组;MALAT1的表达与IFNG的表达呈显著负相关(r=-0.595,P=0.001)。糖皮质激素治疗能显著上调不同亚型CRSwNP中MALAT1的表达(P=0.015)。结论MALAT1在不同亚型CRSwNP中显著低表达,且与IFNG的表达呈负相关,糖皮质激素能上调MALAT1的表达。

Objective To explore the expression of metastasis-associated lung adenocarcinoma transcript 1(MALAT1)in different subtypes of chronic rhinosinusitis with nasal polyps(CRSwNP)and its response to glucocorticoid therapy.Methods Real-time PCR was used to detect the expression of MALAT1 in nasal tissues from eosinophilic CRSwNP,non-eosinophilic CRSwNP and healthy control subjects.The correlation between the expression of MALAT1 and Th1/Th2/Th17 cytokines was analyzed.Moreover,the effect of glucocorticoid treatment on MALAT1 expression was measured.Results MALAT1 was significantly down-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control.Moreover,the expression of MALAT1 was significantly lower in non-eosinophilic CRSwNP than that of eosinophilic CRSwNP.In contrast,interferon gamma(IFNG)was significantly up-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control.The Spearman correlation test showed that the expression of MALAT1 was negatively correlated with the expression of IFNG(r=-0.595,P=0.001).In addition,MALAT1 was significantly up-regulated by glucocorticoid treatment both in patients with eosinophilic CRSwNP and non-eosinophilic CRSwNP(P=0.015,respectively).Conclusion MALAT1 is significantly down-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control,and it negatively correlated with the expression of IFNG.Glucocorticoid treatment significantly up-regulate the expression of MALAT1 in CRSwNP.