RBM24在胃癌发展中的功能研究

Function of RBM24 in the Progression of Gastric Cancer

目的探索胃癌中异常表达的RBM24(RNA-binding motif 24)蛋白对胃癌发展的影响。方法使用慢病毒对RBM24基因进行过表达、siRNA对RBM24基因进行敲低,通过细胞划痕、transwell以及检测肿瘤迁移相关蛋白等实验,研究RBM24对胃癌细胞SGC-7901和MGC-803细胞表型的影响。结果在过表达RBM24后SGC-7901划痕愈合能力和迁移能力降低,E-cad蛋白表达上调;敲低RBM24后,SGC-7901和MGC803细胞划痕愈合能力和迁移能力提高。结论RBM24可能在胃癌发展方面发挥重要作用,为胃癌的早期诊断、预后判断和治疗提供了新思路。

Objective Explored the effects of abnormally expressed RBM24(RNA-binding motif 24)protein on the progression of gastric cancer.Methods Used lentivirus to overexpress the RBM24 gene and siRNA to knock down the RBM24 gene,and studied the effects of RBM24 on the phenotype of SGC-7901 and MGC-803 gastric cancer cells through cell scratching,transwell,and detection of tumor migration-related proteins.Results After overexpression of RBM24,the scratch healing ability and migration ability decreased,E-cad protein expression was up-regulated in SGC-7901 cells.After knocking down RBM24,SGC-7901、MGC-803 cells increased scratch healing ability and migration ability.Conclusion RBM24 may play an important role in the progression of gastric cancer,providing theoretical basis for the diagnosis,prognosis and treatment of gastric cancer.