和中消滞合剂中总槲皮素的定性定量研究

Qualitative and quantitative research on total quercetin of Hezhongxiaozhi mixture

目的建立和中消滞合剂中总槲皮素的定性定量方法。方法采用薄层色谱法定性,高效液相色谱法(HPLC)定量。色谱柱为Inertsil ODS-XP柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.4%磷酸溶液(36∶64),检测波长360 nm,流速为1 ml·min^-1,柱温为30℃。结果薄层色谱斑点清晰;HPLC的槲皮素进样浓度在0.039~0.413μg·ml^-1范围内,与峰面积值呈良好的线性关系,回归方程Y=46589X-4756(r=0.9999);平均加样回收率为98.10%,RSD=0.96%(n=6)。结论建立和中消滞合剂中总槲皮素的定性定量测定方法可行,可用于该制剂的质量控制。

Objective To establish the method for the qualitative and quantitative of total quercetin in Hezhongxiaozhi mixture.Methods TLC was used for qualitative analysis and HPLC for quantitative analysis.Chromatographic conditions were as follows:Inertsil ODS XP(4.6 mm×150 mm,5μm)column was used with mobile phase consisted of methanol 0.4%phosphoric acid(36∶64)at a flow rate of 1.0 ml·min^-1 under a detection wavelength at 360 nm.The column temperature was 30℃.Results TLC spots were clear;the linear range of aristolochic acid was 0.039~0.413μg·ml^-1,and the regression equation was Y=46589X-4756(r=0.9999)with an average recovery rate of 98.10%(RSD=0.96%,n=6).Conclusion The method for the qualitative and the determination of total quercetin can be used for the quality control of Hezhongxiaozhi mixture.