miR-802靶向Hnf1B抑制胰岛素分泌的作用研究

Inhibition of miR-802 on insulin secretion by targeting Hnf1B

探究miR-802对于胰岛β细胞分泌胰岛素的影响及其作用机制。利用miR-802类似物及miR-802抑制剂分别在胰岛原代细胞及Min6细胞过表达或敲降miR-802;采用ELISA法检测miR-802对胰岛素分泌的影响;通过miRNA靶基因数据库预测、荧光素酶报告法和Western blot法确证miR-802的靶基因;最后开展功能回复实验阐明miR-802调控胰岛β细胞分泌胰岛素的作用机制。结果表明:在胰岛原代细胞和Min6细胞中过表达miR-802能抑制胰岛β细胞分泌胰岛素;qPCR和Western blot实验证明miR-802通过抑制靶基因肝细胞核因子1B(hepatocyte nuclear factor 1β,Hnf1B)的转录和翻译,从而抑制胰岛素的分泌。

To investigate the effect of miR-802 on insulin secretion by isletβcells and its mechanism,miR-802 was overexpressed or knocked down in primary islet cells and Min6 cells via transfecting miR-802 mimic and miR-802 inhibitor,respectively.The effect of miR-802 on insulin secretion was detected by ELISA.The target gene of miR-802 was confirmed by miRNA target gene database prediction,luciferase report and Western blot.The function recovery experiment was carried out to clarify the mechanism of miR-802 regulatingβcell secretion of insulin.The results showed that overexpression of miR-802 in islet primary cells and Min6 cells inhibited insulin secretion.qPCR and Western blot showed that miR-802 inhibited insulin secretion by inhibiting the transcription and translation of the target gene,hepatocyte nuclear factor 1β(Hnf1B).