高分子绷带结合塑料杆建立的兔膝骨关节炎模型

Rabbit knee osteoarthritis model established using polymer bandage and plastic rod

背景:目前国内外有多种建立兔膝骨关节炎模型的方法,但大多模型建立中方法较为繁琐,原有的石膏制动方法存在部分缺陷。目的:探索采用高分子绷带结合塑料杆的方法建立兔膝骨关节炎模型。方法:选用雄性新西兰大白兔16只,随机分为模型组和对照组。模型组采用屈曲位高分子绷带固定制动的方法建立兔膝骨关节炎模型,对照组为正常组。模型组膝关节固定8周后去除高分子绷带,取2组兔滑膜、软骨及半月板进行苏木精-伊红染色组织病理学观察,并进行关节软骨Mankin评分。实验方案经新疆医科大学第一附属医院动物伦理委员会批准(批准号为IACUC-20170222026)。结果与结论:①光学显微镜下可见,模型组半月板软骨细胞及基质增多,部分软骨细胞体积增大,软骨陷窝清晰可见;滑膜组织少量慢性炎细胞浸润,胶原纤维增多,部分增粗伴玻璃样变性,部分滑膜组织呈绒毛-结节状增生;部分软骨面有轻微裂隙,软骨细胞及基质轻度增生,少部分软骨细胞及基质减少,胶原纤维排列紊乱。病理结果显示,具有典型的膝骨关节炎病理学改变;②模型组Mankin评分显著高于对照组(P<0.05);③结果说明,采用高分子绷带结合塑料杆能成功建立兔膝骨关节炎模型,局部固定稳定性好,关节角度可控,并且定装置使膝关节完全暴露,局部透气性较好。

BACKGROUND:Various methods have been developed for establishing a rabbit osteoarthritis model worldwide.However,most of modeling methods are cumbersome,and the original gypsum braking method has some defects.OBJECTIVE:To establish the rabbit osteoarthritis model using polymer bandage combined with a plastic rod.METHODS:Sixteen male New Zealand white rabbits were randomly divided into model group and control group.In the model group,the rabbit osteoarthritis model was established through fixed braking using polymer bandage in the flexion position.The control group was normal group.In the model group,the polymer bandage was removed after 8 weeks of fixation.Histological changes of rabbit synovium,cartilage and meniscus were observed using hematoxylin-eosin staining under microscope.Mankin scoring for articular cartilage was performed.The study protocol was approved by the Animal Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University(approval No.IACUC-20170222026).RESULTS AND CONCLUSION:Under the light microscope,the number of meniscus chondrocytes and stroma in the model group was increased,with part of chondrocytes increased in size,and clearly visible cartilage pits.A small amount of chronic inflammatory cells in synovial tissue infiltrated,the number of collagen fibers increased,and some collagen fibers were thickened with hyaline degeneration.Villous-nodular hyperplasia was visible in part of synovial tissues.There were slight fissures on part of the cartilage surface,chondrocytes and stroma slightly proliferated,the number of chondrocytes and stroma reduced partially,and collagen fibers arranged disorderly.The pathological results showed that there were typical pathological changes of knee osteoarthritis in the knee.The Mankin score of the model group was significantly higher than that of the control group(P<0.05).Therefore,the use of polymer bandage combined with plastic rod can successfully establish the rabbit model of knee osteoarthritis,with good local fixation stability,joint angle controllability,and good local permeability.