建立兔胫骨VX2骨肿瘤模型的方法

Establishment of a VX2 bone tumor model in rabbit tibia

背景:关于兔的VX2肿瘤模型方法有多种,但各方法的可靠性无人探究,课题组在原有方法上进行了改良,进行了此项研究。目的:探讨兔VX2肿瘤组织块、细胞悬液分别经改良法与传统植入法进行兔胫骨VX2骨肿瘤造模的可靠程度。方法:将健康成年新西兰大白兔40只随机分为2组,其中组织块组植入肿瘤组织块进行胫骨VX2肿瘤造模,细胞悬液组植入肿瘤细胞悬液进行胫骨VX2肿瘤造模,2组实验动物左侧胫骨均采用穿刺针植入,右侧胫骨均采用传统法植入。造模成功1 h后对穿刺孔周围进行超声检查明确穿刺孔有无血肿,于第3周处死所有实验动物,进行双侧胫骨X射线检查明确肿瘤生长范围,取肿瘤及穿刺部位周围软组织进行大体及病理学检查对比肿瘤大小,并明确有无肿瘤细胞局部浸润。结果与结论:①组织块组有1只实验动物于术后死亡,脱组,细胞悬液组所有实验动物均存活;②X射线检查可见组织块组肿瘤均侵犯皮质层,细胞悬液组皮质层未侵及;肿瘤大体观察可见组织块组肿瘤侵犯骨皮质长度大于细胞悬液组;③组织块组改良法、传统法植入1 h后分别有1例,7例穿刺孔周围血肿;细胞悬液组改良法、传统法植入1 h后分别有2例,9例穿刺孔周围血肿;④组织块组改良法、传统法植入后3个月分别有1例,8例出现肿瘤局部浸润;细胞悬液组改良法、传统法植入后分别有2例,11例出现肿瘤局部浸润;⑤结果表明,组织块植入较细胞悬液植入制作兔VX2骨肿瘤更易培养出大的瘤体,从而使实验更易于观察,且组织块植入后肿瘤局部浸润率较细胞悬液法要低;同时采用改良法植入可有效降低造模过程中肿瘤细胞局部浸润率。

BACKGROUND:Many methods have been developed to establish a rabbit VX2 tumor model,but the reliability of each method has not been explored.In order to develop a reliable method,we made some improvements based on the existing methods.OBJECTIVE:To investigate the reliability of rabbit VX2 tumor tissue block implantation and cell suspension via modified and traditional implantation to make the rabbit tibia VX2 tumor model.METHODS:Forty healthy adult New Zealand white rabbits were randomly divided into two groups.Group A was treated with tissue block implantation for tibia VX2 tumor modeling,and group B was treated with cell suspension for tibia VX2 tumor modeling.Modified and traditional implantation was performed on the left and right tibia of the experimental animals,respectively.One hour after successful modeling,ultrasound examination of the puncture site was performed to determine whether there is hematoma.All experimental animals were sacrificed at 3 weeks.X-ray examination of the bilateral tibia was performed to confirm the tumor growth range.Tumor tissue and soft tissue around the puncture site were taken for general and pathological observation to compare the size of the tumor and identify whether there is tumor cell metastasis.RESULTS AND CONCLUSION:One rabbit died in the tissue block group,and all the experimental animals in the cell suspension group survived.X-ray examination indicated the tumors in the tissue block group invaded the cortex,but the tumors in the cell suspension group did not invade the cortex.Gross observation revealed that the tumor volume of the tissue block group was greater than that of the cell suspension group.In the tissue block group,there were one and seven cases of hematoma around the puncture site at 1 hour after modified and traditional implantation,respectively.In the cell suspension group,there were two and nine cases of hematoma around the puncture site at 1 hour after modified and traditional implantation,respectively.Pathological examination showed that local tumor invasion was found in 1 and 8 cases in the tissue block group as well as in 2 and 11 cases in the cell suspension group at 3 months after modified and traditional implantation,respectively.Our findings indicate that the tissue block implantation method is easier and more convenient than the cell suspension method for making rabbit VX2 bone tumors,and the tumor invasion rate of the tissue block implantation method is lower than that of the cell suspension method.Improved tissue block implantation can effectively reduce the tumor invasion rate during modeling.