一测多评纽斯葆牌多种维生素矿物质片中维生素D3和维生素E的含量

A Quantitative Method for Simultaneous Assay of vitamin D3 andVitamin Ein NuspowerMultivitamin & Mineral Tablets

目的:建立一测多评高效液相色谱法检测纽斯葆牌多种维生素矿物质片中维生素D3和维生素E的含量。方法:取样品适量,经皂化、提取、净化、浓缩后,采用高效液相色谱法通过一次前处理一次进样同时测定维生素D3和维生素E,并用加标回收试验校正含量测定结果。结果:维生素D3和维生素E在20 min内完全分离,维生素D3浓度在0.15~0.87μg·mL-1的范围内,维生素E在2.89~10.13 mg·mL-1的范围内,均与对应的峰面积呈良好的线性关系。检测结果与现行国家标准方法检测结果基本一致。维生素D3检出限为0.0056μg·mL-1,维生素E的检出限为0.0119 mg·mL-1。结论:该方法简单、高效,检测成本低,适用于该产品中维生素D3和维生素E含量的快速测定。

Objective:To establish a quantitative method for simultaneous assay of vitamin D3 and vitamin Ein Nuspower Multivitamin&mineral tablets.MethodThe contents of vitamin D3 and vitamin E were detected through one injection by HPLC after one pretreatment including saponification,extraction,purification and concen-tration,andcalibration result by Standard Recovery Test.Results:Vitamin D3 and vitamin E we-re separated within 20 min.VitaminD3 concentration was good liner with peak area in the ran-g of 0.15~0.87μg·mL-1 and vitamin E concentration was good liner with peak area in the rang of 2.89~10.13 mg·mL-1.The results are basically consistent with those of theoretical content and current national standard method.The detection limits of vitamin D3 and vitamin E were 0.0056μg·mL-1 and 0.0119 mg·mL-1,respectively.Conclusion:With the advantages of simple operation,high efficiency,low cost,the method is suitable for the rapid determination of Vitamin D3 and vitamin E in this product.