刨花润楠根系和根际土壤真菌群落多样性分析

Analysis of Fungal Community Diversity in Machilus pauhoi Roots and Rhizosphere Soil

为了探究刨花润楠Machilus pauhoi根系和根际土壤真菌多样性及群落结构特征,应用高通量测序技术对20年生的刨花润楠根系、根际土壤和非根际土壤真菌分别进行测序分析。结果表明:共获得1563个真菌OTUs,刨花润楠根系真菌丰富度和多样性指数均低于根际土壤,且非根际土壤最高,但3者间差异不显著(P>0.05)。子囊菌门Ascomycota、担子菌门Basidiomycota、球囊菌门Glomeromycota、壶菌门Chytridiomycota和毛霉菌亚门Mucoromycotina是排名前5的优势菌群,且前3者的相对丰度分别在非根际土壤、根际土壤和根系中占比最高。丰度聚类分析显示,非根际土壤和根际土壤的相似度较高,聚为一类,两者与刨花润楠根系间的相似度相对较低。结论:种植刨花润楠可影响根际真菌菌群组成与丰度大小,土壤真菌分布存在明显的根际效应。

To understand the diversity and community structure of fungi in Machilus pauhoi roots and rhizosphere soil,high-throughput sequencing is used to analyze the sequencing of fungi in 20-year-old M.pauhoi roots,rhizosphere soil,and non-rhizosphere soil.The results show that 1563 OTUs are obtained;although the richness and diversity index of fungi in M.pauhoi roots are lower than that of rhizosphere soil and the highest index is to be found in non-rhizosphere soil,there is no significant difference among the three(P>0.05).The dominant flora in the top five are Ascomycota,Basidiomycota,Glomeromycota,Chytridiomycota and Mucoromycotina.The relative abundance of the first three species has accounted for the highest proportion in non-rhizosphere soil,rhizosphere soil and M.pauhoi roots respectively.Abundance cluster analysis shows that non-rhizosphere soil and rhizosphere soil have higher similarity,clustering into one group whereas the similarity between them and the roots of M.pauhoi is relatively low.To conclude,the composition and abundance of rhizosphere fungi are significantly affected by the cultivation of M.pauhoi,and there is an obvious rhizosphere effect on the distribution of soil fungi.