不同蛋白酶对灰树花蛋白的水解度及水解产物的抗氧化活性

Hydrolysis Degree of Different Proteases to the Protein of Grifola Frondosa and the Antioxidant Activity of the Hydrolysates

为明确不同蛋白酶对灰树花蛋白的水解度及水解产物的抗氧化活性,采用胃蛋白酶、胰蛋白酶、胃蛋白酶+胰蛋白酶、碱性蛋白酶、木瓜蛋白酶分别水解灰树花蛋白,并测定不同蛋白酶对灰树花蛋白水解产物的抗氧化活性。结果表明:5种蛋白酶对灰树花蛋白的水解度由高到低的顺序依次为碱性蛋白酶>胃蛋白酶+胰蛋白酶>胃蛋白酶>胰蛋白酶>木瓜蛋白酶,以碱性蛋白酶的水解度最高,水解度达35.48%;碱性蛋白酶、胃蛋白酶、胃蛋白酶+胰蛋白酶的水解产物抗氧化活性均随着浓度的增加而提高,相同浓度下3种蛋白酶中以碱性蛋白酶水解产物的抗氧化活性最强,当其浓度为0.5 mg·mL^-1和1.0 mg·mL^-1时,对DPPH自由基的清除率达90%以上,与对照Vc相当。

In order to determine the hydrolysis degree of different proteases to the protein of Grifola frondosa and the antioxidant activity of the hydrolysates,the pepsin,trypsin,pepsin+trypsin,alkaline protease and papain were used to hydrolyze the protein of Grifola frondosa respectively,and the antioxidant activity of different proteases to the hydrolysates of the protein of Grifola frondosa was determined.The results showed that the hydrolysis degree of the five proteases to the protein of Grifola frondosa was from high to low in the order of alkaline protease>pepsin+trypsin>pepsin>trypsin>papain,and the hydrolysis degree of alkaline protease was the highest,reaching 35.48%.The antioxidant activity of the hydrolysates of alkaline protease,pepsin and pepsin+trypsin increased with the increase of concentration,and the antioxidant activity of the hydrolysates of alkaline protease was the strongest among the three proteases at the same concentration,when the concentration was 0.5 mg·mL^-1 and 1.0 mg·mL^-1,the clearance rate of DPPH free radical was more than 90%,which was similar to that of the control Vc.