改良小鼠胚胎下颌下腺的获取及其体外器官培养模型的建立

Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model

分支形态的形成对于保证器官可以在有限的体积内获得最有效的功能形态具有重要意义。下颌下腺是研究器官分支形态发生过程的重要模型,在小鼠胚胎中获取下颌下腺组织进行体外器官培养是一种重要的研究方法。本文介绍了一种改良的小鼠胚胎下颌下腺获取方法,并将整个获取及建立体外器官培养流程作以简介,以对其在体内发育过程中的分支形态发生过程进行更好体外的模拟,从而能够更好地开展相关研究。

Branching morphology is important to ensure that the organ can obtain the efficient functional morphology in a limited volume.The submandibular gland is a crucial model for studying the morphological processes of organ branches.Harvesting the submandibular gland from mouse embryo is also an essential research technique.In this paper,a modified method for obtaining the submandibular glands of mouse embryo was introduced,and the whole process of obtaining and establishing in vitro organ culture was briefly introduced to accurately simulate branch morphogenesis for vivo development and related research.