Hyperactive reactive oxygen species impair function of porcine Sertoli cells via suppression of surface protein ITGB1 and connexin-43

Hyperactive reactive oxygen species impair function o porcine Sertoli cells via suppression of surface protein ITGB1 and connexin-43

DEAR EDITOR,Gap junctions regulate intercellular communication between Sertoli cells and germ cells in male testes and play vital functions in spermatogenesis.Many factors in animal breeding and husbandry can induce oxidative stress,which can impair the testis microenvironment and male animal fertility.However,the underlying mechanisms are largely unknown.Recently,we identified that androgen signals promote the expression of connexin-43(Cx43),a key component of gap junctions,to regulate spermatogenesis.Thus,we asked whether hyperactive reactive oxygen species(ROS)can impair gap junctions by interfering with Cx43 in porcine testes.Using a porcine Sertoli cell in vitro system,we found that hyperactive ROS caused extensive apoptosis in Sertoli cells,remarkable decrease in Cx43 expression,and failed maintenance of co-cultured spermatogonial stem cells(SSCs),indicating that ROS impaired the function of Sertoli cells and promoted loss of SSCs.This observation provides a possible mechanism for the impact of ROS on fertility of male animals.