水苏碱对胃癌移植瘤小鼠肿瘤生长及基因表达的影响

Effect of Stachydrine on Tumor Growth and Gene Expression in Gastric Xenograft Cancer in Mice

【目的】探讨水苏碱对胃癌移植瘤裸鼠肿瘤生长及CyclinD1、c-myc、Bcl-2、c-Met、p53、p16、Bax、Caspase-3表达的影响。【方法】培养人胃癌SGC-7901细胞株后制备细胞悬液,将细胞悬液接种至BALB/c裸鼠皮下得到胃癌移植瘤小鼠模型,随机分为用生理盐水灌胃的对照组、用水苏碱(stachydrine,STA)灌胃的STA组。干预后21d,测定移植瘤体积、重量及移植瘤中上述癌基因的表达。【结果】STA组的移植瘤体积、重量均明显低于对照组;移植瘤中cyclinDl、c-myc、Bcl-2、c-Met的蛋白表达量均明显低于对照组,而p53、p16、Bax、Caspase-3的蛋白表达量均明显高于对照组。【结论】水苏碱能够抑制胃癌移植瘤小鼠的肿瘤生长并使移植瘤中的原癌基因表达下调、抑癌基因表达上调。

【Objective】To study the regulation effect of stachydrine on tumor growth and the expression of CyclinD1,c-myc,Bcl-2,c-M et,p53,p l6,Bax,Caspase-3 in gastric cancer xenograft tumors m ice.【Methods】After cultured SGC-7901 cell line of human gastric cancer,the cell suspension was inoculated into BALB/C nude mice subcutaneously to obtain the transplanted tumor model of gastric xenograft cancer.The mice were randomly divided into two groups:the control group treated with normal saline and the STA group treated with stachydrine.The volume and weight of the transplanted tumor and the expression of these oncogenes were measured after 21 days.【Results】The volume and weight of the transplanted tumor in the STA group were significantly lower than those in the control group;the protein expression of CyclinDl,c-myc,Bcl-2 and c-Met in the transplanted tumor was significantly lower than that in the control group,while the protein expression of p53,p i6,Bax and Caspase-3 were significantly higher than that in the control group.【Conclusion】Stachydrine can inhibit the growth of gastric cancer transplanted tumor mice,down regulate the expression of proto oncogene and up regulate the expression of tumor suppressor gene.