爵床提取物抗炎镇痛作用的实验研究

EXPERIMENTAL STUDIES ON THE ANTI-INFLAMMATORY AND ANALGESIC EFFECT OF ROSTELLULARIA PROCUMBENS EXTRACT

目的探讨爵床提取物的抗炎镇痛作用。方法采用致炎剂二甲苯引起小鼠耳肿胀、新鲜蛋清引起大鼠足跖肿胀2种急性炎症模型以及棉球引起大鼠肉芽肿慢性炎症模型,研究爵床提取物灌胃给药对急慢性炎症的作用。采用热板法、醋酸扭体法和甲醛法致痛,研究爵床提取物灌胃给药的镇痛作用。结果与纯化水对照组相比,爵床醇提取物和水提取物高、低剂量组对致炎剂二甲苯所引起的小鼠耳廓肿胀抑制显著(P<0.01,P<0.05),对蛋清所引起的大鼠足跖肿胀抑制显著(P<0.01),对大鼠棉球肉芽肿抑制显著(P<0.01)。爵床醇提取物和水提取物高、低剂量组给药后对热板所引起的小鼠痛阈值明显提高,与纯化水对照组和自身给药前比较差异明显(P<0.01,P<0.05);爵床醇提取物和水提取物高、低剂量组对致痛剂醋酸所引起的小鼠扭体反应次数明显减少,也明显抑制甲醛所引起的早期(0~5 min)和晚期(15~30 min)小鼠足痛反应,与纯化水对照组比较差异显著(P<0.01)。爵床醇提取物的抗炎镇痛作用比水提取物强(P<0.01,P<0.05)。结论爵床提取物灌胃给药具有一定抗炎、镇痛作用,且醇提取物强于水提取物。

Objective:To investigate the anti-inflammatory and analgesic effects of Rostellularia procumbens extract.Methods:The anti-inflammatory animal models were used in researching the effect of R.procumbens extract on acute and chronic inflammation.The acute models were xylene-induced ear edema and egg white-induced paw edema,while the chronic one was granuloma induced by cotton pellet implantation.Meanwhile,the analgesic effect of the extract was observed by hot plate acetic,acid writhing test and the formalin induced-pain experiment.Results:Compared with the purified water control group,high and low doses of R.procumbens alcohol and water extract could significantly inhibit ear edema in mice(P<0.01,P<0.05)and the paw edema in rat(P<0.01).Also,the alcohol and water extract decreased the granuloma of rats(P<0.01).Moreover,the pain threshold with hot plate method was significantly increased(P<0.01,P<0.05)compared with the purified water control group and the data before the drugs administration of high and low doses of R.procumbens alcohol and water extract.And the writhing number was reduced(P<0.01)when contrasted to the purified water control group after extract administration.The alcohol and water extract also significantly inhibited foot pain response at early phase(0-5 min)and late phase(15-30 min)caused by formalin in mice(P<0.01).However,there were substantial variations in the alcohol extract groups and the same dose of water extract in high and low concentrations(P<0.01,P<0.05).Conclusion:R.procumbens extract possesses the anti-inflammatory and analgesic effect,alcohol extract has better effect than water extract.