化香果提取物体外抑菌活性研究

In Vitro Antibacterial Activity of Extract from Platycarya strobilacea Sieb.et Zucc.

研究了化香果提取物对水产常见9种致病菌嗜水气单胞菌(Aeromonas hydrophila)、温和气单胞菌(Aeromonas sobria)、豚鼠气单胞菌(Aeromonas caviae)、维氏气单胞菌(Aeromonas veronii)、迟钝爱德华菌(Edwardsiella tarda)、副溶血弧菌(Vibrio parahaemolyticus)、鼠疫耶尔森氏菌(Yersinia pestis)、大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)的抑菌活性。研究结果表明:化香果提取物对9种细菌均具有抑制作用,最小抑菌浓度为4.50 g/L。对每种细菌而言,抑菌效果随化香果提取物质量浓度的增加而增强,其中对大肠杆菌的抑制能力最强,IC_(50)值为0.035 g/L;金黄色葡萄球菌次之,IC_(50)值为0.053 g/L。对雌雄小鼠急性经口毒性试验结果表明化香果提取物对小鼠的急性经口半数致死量(LD_(50)值)分别为5 010和7 940 mg/kg体质量,表明化香果提取物属于实际无毒级。

The antibacterial activity of the extract from Platycarya strobilacea(PSE) was measured in vitro against nine bacteria including Aeromonas hydrophila,Aeromonas sobria,Aeromonas caviae,Aeromonas veronii,Edwardsiella tarda,Vibrio parahaemolyticus,Yersinia pestis,Escherichia coli and Staphylococcus aureus. The results showed that PSE had inhibitory effects on 9 kinds of bacteria, and the minimum inhibitory concentration was 4.50 g/L. For each type of bacteria, the bacteriostatic effect was enhanced with the increase of PSE concentration, in which the strongest antibacterial activity was against E. coli with the IC50 of 0.035 g/L, followed by Staphylococcus aureus with the IC50 of 0.053 g/L. The results of imported toxicity test on mice showed that the LD50 values of PSE to male and female mice were 5 010 and 7 940 mg/kg weight, which indicated that the PSE was actually non-toxic.