沙棘果油微胶囊化制备工艺的优化及其表征

Optimization and Characterization of Microencapsulation Technology for Seabuckthorn Fruit Oil

为研究混合壁材及乳化剂对沙棘果油微胶囊包埋效果及稳定性的影响,本文以沙棘果油为芯材,以酪蛋白酸钠和变性淀粉(辛烯基琥珀酸淀粉钠)为混合壁材,单双硬脂酸甘油酯为乳化剂,麦芽糊精为填充剂,柠檬酸钾为缓冲盐,通过喷雾干燥法制备50%载油率的沙棘果油微胶囊。在单因素实验的基础上,以酪蛋白酸钠添加量、变性淀粉添加量、乳化剂添加量为实验因素,采用Box-Behnken法设计三因素三水平试验进行优化,确定微囊粉的最佳包埋效果。结果表明,所得的回归模型具有高度的显著性(P<0.0001),方程对试验拟合较好,在酪蛋白酸钠添加量8%、变性淀粉添加量16%、乳化剂添加量2%条件下,制备得到的沙棘果油微囊粉包埋率最大,可以达到91.6%;扫描电镜观察微胶囊粉表面圆润无裂痕,马尔文激光粒度分析仪测定复原乳粒径<800 nm,通过油脂氧化自动分析仪进行油脂氧化诱导期分析,对照组沙棘果油氧化诱导期为7 h,试验组沙棘果油微囊粉氧化诱导期为40 h,表明沙棘果油微囊粉稳定性强,不易氧化变质,货架期较长。

In order to study the effect of mixed wall materials and emulsifiers on the embedding effect and stability of seabuckthorn fruit oil microcapsules,the seabuckthorn fruit oil was used as core material,with sodium caseinate and modified starch(octyl succinic acid sodium starch)as mixed wall material,single and double stearic acid glyceride as emulsifier,maltodextrin as filling agent,potassium citrate as buffer salt,and seabuckthorn oil microcapsules with 50%oil loading rate prepared by spray drying method.Based on the single factor experiment,taking the content of sodium caseinate,modified starch and emulsifier as the experimental factors,box Behnken method was used to design three factors and three level experiment to optimize the response surface and determine the best embedding effect.The results showed that the regression model was highly significant(P<0.0001).The equation fitted the experiment well.Under the conditions of 8%sodium caseinate,16%modified starch and 2%emulsifier,the embedding rate of seabuckthorn oil microcapsule powder was the highest,which could reach 91.6%.The surface of microencapsulated powder was round and free of cracks by SEM.The particle size of reconstituted milk was less than 800 nm by Malvern laser particle size analyzer.The induction period of oil oxidation was analyzed by oil oxidation automatic analyzer.The oxidation induction period of seabuckthorn fruit oil in the control group was 7 h,and the oxidation induction period of seabuckthorn fruit oil microcapsule powder in the test group was 40 h,indicating that seabuckthorn fruit oil microcapsule powder had strong stability,was not easy to oxidize and deteriorate,and has a long shelf life.