裂叶荨麻体外降糖活性化学成分

Chemical Constituents of Hypoglycemic Activity in Vitro from Urtica fissa

目的:研究裂叶荨麻体外降糖活性及化学成分。方法:利用活性追踪法,通过α-葡萄糖苷酶抑制作用比较裂叶荨麻根、茎、叶体外降糖活性,比较根不同极性溶剂萃取相的体外活性。通过硅胶色谱、重结晶进行分离纯化,根据理化性质及波谱数据鉴定化合物结构,再评价其体外活性成分。结果:裂叶荨麻根乙醇提取物的乙酸乙酯相体外降糖活性最强,IC50值为8.499 mg/mL,从中分离出6个化合物,分别鉴定为β-谷甾醇、对羟基肉桂酸甲酯、正十六烷酸、对甲氧基苯甲醛、反式-对羟基肉桂酸、胡萝卜苷。化合物反式-对羟基肉桂酸和胡萝卜苷对α-葡萄糖苷酶的抑制活性较强,其IC50值分别为5.234、1.693 mg/mL。结论:裂叶荨麻根乙醇提取物的乙酸乙酯相中的化学成分体外降糖活性较为明显。

Objective:To study the hypoglycemic activity in vitro and chemical constituents of Urtica fissa.Methods:In vitro hypoglycemic activities of roots,stems and leaves of Urtica fissa and the different polar solvent extraction phase of roots were compared by activity tracing method through inhibition ofα-glucosidase.The compounds were isolated and purified by silica gel chromatography and recrystallization.The structures of compounds were identified according to physicochemical properties and spectral data,and the hypoglycemic of compounds were evaluated.Results:The ethyl acetate phase of ethanol extract in the roots of Urtica fissa showed the most significant in vitro hypoglycemic activity with the IC50 value of 8.499mg/mL.Six compounds were isolated and identified asβ-sitosterol,methyl p-hydroxycinnamate,n-hexadecanoic acid,p-methoxybenzaldehyde,trans-phydroxylcinnamic acid and carotenoid.Trans-p-hydroxylcinnamic acid and carotenoid exhibited significant inhibitory activities againstα-glucosidase with the IC50 values of 5.234 and 1.693 mg/mL.Conclusion:The chemical constituents in the ethyl acetate phase of the ethanol extract from the root of Urticaria lobata had obvious hypoglycemic activity in vitro.