石斛粉葛咀嚼片的制备及其抗氧化活性

Preparation and Antioxidant Activity of a Kind of Chewable Tablets with Dendrobium officinale and Pueraria thomsonii

以石斛和粉葛为原料,经超微粉碎、配料、湿法造粒、干燥、整粒、压片等工序制成咀嚼片。考察了原料比、辅料比、乙醇浓度、60%乙醇添加量、硬脂酸镁添加量对咀嚼片感官品质的影响,在单因素实验基础上,采用L16(45)正交试验优化咀嚼片配方;以DPPH·清除能力、ABTS+·清除能力、总抗氧化能力为模型评价咀嚼片抗氧化活性。结果表明,石斛粉葛咀嚼片最佳工艺配方为:原料中石斛∶粉葛为1∶4,原辅料比[(石斛+粉葛):辅料(玉米淀粉+菊粉+木糖醇+甘露醇)]为1∶1.5,辅料配比(玉米淀粉∶菊粉∶木糖醇∶甘露醇)为4∶3∶1∶1,润湿剂60%乙醇的添加量为0.4 mL/g,硬脂酸镁1.5%,咀嚼片感官评分为93.7±0.03分。石斛粉葛咀嚼片水提液、石斛粉葛咀嚼片醇提液与抗坏血酸(VC)的清除DPPH·的IC50值分别为(3.067±0.1095)、(3.512±0.0945)、(0.06337±0.0286)mg/mL;石斛粉葛咀嚼片水提液、石斛粉葛咀嚼片醇提液与VC的清除ABTS+·的IC50值分别为(5.529±0.8175)、(5.380±0.7915)、(0.2772±0.0345)mg/mL;石斛粉葛咀嚼片水提液、醇提液、VC在浓度为2 mg/mL时,总抗氧化能力分别为0.258、0.188、0.494。石斛粉葛咀嚼片有一定的抗氧化活性,石斛与粉葛有协同抗氧化作用。

Using Dendrobium officinale and Pueraria thomsonii as main materials while with corn starch,inulin,xylitol and mannitol as excipient,chewable tablets were prepared by ultrafine grinding,ingredients,wet granulation,drying,whole grain,pressing tablets and so on. The effect of ratio of main materials to excipient,proportion of auxiliary materials,ethanol concentration,adding amount of 60% ethanol and additive amount of magnesium stearate on the sensory quality of chewable tablet were observed. The formula of chewable tablets was optimized by L16(45)orthogonal experiment based on single experiment. The antioxidant activity of chewable tablets was evaluated by the model of DPPH· scavenging ability,ABTS+· scavenging ability and total antioxidant capacity.The results showed that the optimum technological formula were as follows:The ratio of main materials to excipient was 1∶1.5 while the ratio of Dendrobium officinale to Pueraria thomsonii was 1∶4,the proportion of auxiliary materials that was corn starch:inulin:xylitol:mannitol were 4∶3∶1∶1,wetting agent of 60% ethanol 0.4 mL/g,magnesium stearate 1.5%,and under these conditions,sensory evaluation scores of the product was 93.7±0.03 score. The scavenging ability of chewable tablets water extract,chewable tablets alcohol extract and ascorbicacid VC against DPPH· were(3.067±0.1095),(3.512±0.0945)and(0.06337±0.0286) mg/mL while against ABTS+· were(5.529±0.8175),(5.380±0.7915)and(0.2772±0.0345) mg/mL,respectively. The total antioxidant capacity of chewable tablets water extract,chewable tablets alcohol extract and ascorbic acid VC with the concentration of 2 mg/mL were 0.258,0.188 and 0.494,respectively. Furthermore,all of the antioxidant activities mentioned above showed a dose relationship. The chewable tablets with Dendrobium officinale and Pueraria thomsonii would have a certain antioxidant activity and synergistic effect of Dendrobium officinale and Pueraria thomsonii in terms of antioxidant activity.