壳寡糖复合固体饮料对Ⅱ型糖尿病小鼠肠道菌群结构的影响

Effect of Chitooligosaccharide Compound Solid Beverage on Intestinal Flora of Type II Diabetic Mice

探究壳寡糖复合固体饮料(壳糖平)对糖尿病模型小鼠肠道菌群结构变化的影响。将70只小鼠随机分为正常组(Normal feeding group,NFD)、模型组(Type 2 diabetes group,T2DM)、阿卡波糖组(Acarbose group,Acar)、壳寡糖组(Chitooligosaccharide group,COS)、壳糖平低剂量组(Ke Tang Ping low dose group,LKTP)、壳糖平中剂量组(Ke Tang Ping medium dose group,MKTP)、壳糖平高剂量组(Ke Tang Ping high dose group,HKTP),灌胃4周后,取结肠内容物进行肠道菌群分析。结果表明,各组的16S测序有效序列范围为130301~244496条,其长度范围在315~478 bp,OTU范围为1367~1835,物种覆盖率均大于0.99。韦恩图分析表明,有556个OTU是7组共有的,其中326(NFD组)、223(T2DM组)、237(Acar组)、118(COS组)、197(LKTP组)、142(MKTP组)、181(HKTP组)个特有的;PCoA图表明NFD、T2DM、Acar、COS、LKTP、MKTP和HKTP组组内样品具有良好的平行性和重复性;与T2DM组相比,COS组的处理效应弱,Acar、LKTP、MKTP和HKTP组处理效应强。与模型组相比,在门水平,灌服壳糖平可显著增加T2DM小鼠肠道Bacteroidetes的相对丰度而降低Firmicutes的相对丰度;在科水平,灌服壳糖平可显著增加T2DM小鼠肠道Bacteroidales_S24-7_group、Prevotellaceae的相对丰度,而Lachnospiraceae和Desulfovibrionaceae的相对丰度显著下降,其中壳糖平中、高剂量组效果最显著。结论:壳糖平可以影响肠道菌群的组成和多样性,并且促进有益菌的生长与定植,降低条件致病菌的相对丰度,从而促进肠道健康。

To investigate the effects of chitoloigosaccharides compound solid beverage(Ke Tang Ping)on intestinal microbiota of mice with type 2 diabetes,the mice were randomly divided into seven groups,including the normal group(NFD),the model group(T2DM),the Acarbose group(Acar),chitooligosaccharides group(COS),and Ke Tang Ping low dose group(LKTP),Ke Tang Ping medium dose group(MKTP),Ke Tang Ping high dose group(HKTP),respectively.After 4 weeks of administration,the intestinal contents were taken for intestinal flora analysis.The results indicated that the effective sequence of 16S sequencing of each group ranged from 130301 to 244496 reads,and its length ranged from 315 to 478 bp,OTU range was 1367~1835,the species coverage bacteria was greater than 0.99.The Venn diagram indicated that 556 OTUs were shared by 7 groups.Among them,326(NFD group),223(T2DM group),237(Acar group),118(COS group),197(LKTP group),142(MKTP group),181(HKTP group)were unique.The PCoA plots showed good parallelism and repeatability of samples in the NFD,T2DM,Acar,COS,LKTP,MKTP,and HKTP groups.Compared with the T2DM group,the COS group had a weak treatment effect,while the Acar,LKTP,MKTP and HKTP groups had strong treatment effects.Compared with the model group(T2DM),at the phylum level,when the mice were fed Ke Tang Ping,the relative abundance of Bacteroidetes increased,and the relative abundance of Firmicutes decreased.At the family level,the relative abundance of Bacteroidales_S24-7_group and Prevotellaceae increased significantly fed with Ke Tang Ping.However,the relative abundance of Lachnospiraceae and Desulfovibrionaceae decreased significantly.In conclusion,Ke Tang Ping could change the composition and diversity of gut microbes,promote the colonization of beneficial bacteria and reduce the relative abundance of opportunistic pathogens to promote intestinal health,and assist in the treatment of diseases such as type 2 diabetes.