摘要
[目的]提高假单胞菌的增殖,降低生产成本。[方法]采用光电比浊法,通过单因素试验对假单胞菌增殖培养基的碳源和氮源进行优化。[结果]假单胞菌增殖培养基中最佳的碳源、氮源种类与浓度分别为蔗糖0.5 g/L、鱼蛋白胨1.2 g/L。[结论]通过对增殖培养基的优化,降低了生产成本。
[Objective]To improve the proliferation of Pseudomonas and reduce the production cost.[Method]The photoelectric turbidimetry method was used to optimize the carbon and nitrogen sources of Pseudomonas enrichment medium by single factor test.[Result]The optimal carbon source and nitrogen source species and concentration in Pseudomonas enrichment medium were sucrose 0.5 g/L and fish protein 1.2 g/L,respectively.[Conclusion]By optimizing the enrichment medium,the production cost was reduced.
作者
宫达非
廖静云
曾慧帆
尚常花
GONG Da-fei;LIAO Jing-yun;ZENG Hui-fan(College of Life Science,Guangxi Normal University,Guilin,Guangxi 541006;Key Laboratory of Ecology and Environmental Protection of Rare and Pndangered Animals and Plants,Ministry of Education,Guilin,Guangxi 541006)
出处
《安徽农业科学》
CAS
2020年第7期83-84,88,共3页
Journal of Anhui Agricultural Sciences
基金
广西高校中青年教师基础能力提升项目(2018KY0086)
广西师范大学博士科研启动基金项目(2017BQ015)
广西研究生教育创新计划项目(JGY2019009)。
关键词
假单胞菌
碳源
氮源
优化
Pseudomonas
Carbon source
Nitrogen source
Optimization
