酶标仪在艾滋病血液检验上的应用效果

Effect of Enzyme Labeling Apparatus on Blood Test of AIDS

目的:探究艾滋病血液检验过程中应用酶标仪的临床价值。方法:给予临床化学检验规范操作流程的前提下,设置相应的工作程序,对采血、检验与超微量化学检验等整个过程充分完成,分析血液检验中应用酶标仪的临床效果。结果:实验中选择艾滋病为例,便于血液检测仪器更具针对性,对3份血清予以批内与批间检测,反复检测20次,与血液分析仪相比,酶标仪批内与批间精密度更高(P<0.05);选择艾滋病血清作为试验标本,结果证实线性范围临界值160卡门单位,在艾滋病活性实现上述标准后可借助等渗盐水予以稀释,并完成检查操作;混合后根据艾滋病方法检查,结果显示:血液分析仪平均回收率94.36%,低于酶标仪的98.17%(P<0.05);将氢氧化钠添加到1份艾滋病血清标本中,浓度0.4mmol/L,显色后的不同时间予以比色处理,结果显示:10~90min的显色相对较稳定,显色10min后的比色情况比较好。结论:酶标仪具有稳定性、简便性、准确性以及快速性等特点,对艾滋病的检验效果显著。

Objective:To explore the clinical value of enzyme-linked immunosorbent assay(ELISA)in the blood test of AIDS.Methods:On the premise of standardizing the operation process of clinical chemical examination,the corresponding working procedures were set up to fully complete the whole process of blood collection,examination and ultra-micro chemical examination,etc.The clinical effect of using enzyme-labeled instrument in blood examination was analyzed.Results:AIDS was selected as an example in the experiment,which was convenient for blood testing instruments to be more targeted.three serum samples were tested intrabatch and intrabatch for 20 times repeatedly.compared with blood analyzer,microplate reader had higher intra-batch and intrabatch precision(P<0.05).Sodium hydroxide was added to a serum sample of AIDS at a concentration of 0.4mmol/L,and the color was compared at different times after color development.The results showed that the color development was relatively stable between 10 and 90 minutes,and the color comparison after 10 minutes was better.Conclusion:Enzyme-linked immunosorbent assay has the characteristics of stability,simplicity,accuracy and rapidity,and has a significant effect on the detection of AIDS.