抗癌防移片对BALB/c小鼠大肠癌肝转移HGF、VEGF蛋白表达的影响

Effect of Anti-cancer and Anti-migration Tablets on HGF,VEGF Expression in Liver Metastasis of Colorectal Cancer in BALB/c Mice

目的:探究抗癌防移片对于抑制大肠癌肝转移的作用及其机制。方法:采用BALB/c小鼠注入CT26细胞悬液,建立大肠癌小鼠模型,造模成功后的48只小鼠随机分为4组:模型组,西药组,中药组,中西药组,每组12只小鼠,分别予以不同的处理方式干预4周。采用免疫组织化学测定HGF、VEGF蛋白表达光镜下观察,对HGF、VEGF蛋白进行分析比较。结果:模型组与西药组、中西结合组比较,HGF、VEGF蛋白表达明显升高(P<0.01);单纯中药组与西药组、中西结合组相比,HGF、VEGF蛋白表达明显升高(P<0.05);中西结合组与西药组比较,HGF、VEGF蛋白表达下降(P>0.05)。结论:抗癌防移片可能通过抑制HGF、VEGF的表达,抑制血管新生,从而抑制大肠癌肝转移。

Objective:To investigate the effect of anti-cancer and anti-migration tablets on inhibiting liver metastasis of colorectal cancer and its mechanism.Methods:BALB/c mice were injected with CT26 cell suspension to establish a model of colorectal cancer in mice.Fortyeight mice were randomly divided into four groups,twelve mice in each group:model group,western medicine group,traditional Chinese medicine group and Western medicine group.Immunohistochemistry was used to detect the expression of HGF,VEGFprotein under light microscopy,and HGF,VEGF protein was analyzed and compared.Results:The expression of HGF,VEGFprotein in model group was significantly higher than that in western medicine group and integrated Chinese and Western medicine group(P<0.01);the expression of HGF,VEGF protein in simple Chinese medicine group was significantly higher than that in western medicine group and integrated Chinese and Western medicine group(P<0.05);and the expression of HGF,VEGF protein in combined Chinese and Western medicine group was significantly lower than that in western medicine group(P>0.05).Conclusion:Anti-cancer and anti-migration tablets may inhibit hepatic metastasis of colorectal cancer by inhibiting the expression of HGF,VEGFand angiogenesis.