植物-土壤反馈对刺五加幼苗抗氧化酶系统的影响

Effect of Plant and Soil Feedback on Antioxidant System of Acanthopanax senticosus

目的:该文研究了植物土壤反馈对刺五加幼苗抗氧化酶系统的影响,有利于阐述植物-土壤反馈对于刺五加幼苗生长中抗氧化酶系统的变化,为揭示植物-土壤反馈的原因提供理论依据。方法:通过温室盆栽试验,分别对未种植过刺五加的土壤(1组),连续3年种植刺五加的土壤(2组)和多年种植刺五加的土壤(3组)进行株高,叶色值(SPAD),抗氧化酶系统相关指标[蛋白质,超氧化物歧化酶(SOD),过氧化氢酶(CAT),过氧化物酶(POD),抗坏血酸过氧化物酶(APX),丙二醛(MDA)]和产量、生长相关指标进行测定。结果:未种植过刺五加的土壤(1组),连续三年种植刺五加的土壤(2组)和多年种植刺五加的土壤(3组)在后续种植的刺五加幼苗的株高,叶色值(SPAD),生物量,蛋白,SOD,CAT,POD,APX,MDA有显著性差异,ACP活性无显著性差异。其中未种植过刺五加的土壤(1组)的刺五加幼苗MDA,CAT,POD和SOD要高于种植过刺五加的土壤(2,3组)的刺五加幼苗,而蛋白和APX要低于种植过刺五加的土壤(2,3组)的刺五加幼苗。结论:植物和土壤在刺五加幼苗生长过程中呈现负反馈调节,降低幼苗抗氧化酶活性。因此,未种植过刺五加的土壤对刺五加幼苗的生长更有优势。研究结果为阐述植物-土壤反馈对刺五加生长的影响提供研究基础,并为农田栽培刺五加技术标准提供理论依据和技术支持。

Objective:To study the effect of plant-soil feedback on the antioxidant enzyme system of Acanthopanax senticosus seedlings,in order to elucidate the changes of plant-soil feedback on the antioxidant enzyme system of A.senticosus seedlings,and provide theoretical basis for revealing the reasons of plant-soil feedback.Method:Through the greenhouse pot experiment,plant height,leaf color value(SPAD),antioxidant enzymes[protein,superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),aseorbateperoxidase(APX),malondialdehyde(MDA)]and yield and growth related indexes of soil without A.senticosus(group 1),soil with A.senticosus(group 2)for three consecutive years and soil with A.senticosus(group 3)for many years were measured respectively.Result:There were significant differences in plant height,SPAD,protein,SOD,CAT,POD,APX and MDA between seedlings of A.senticosus planted for three consecutive years(group 1),two successive years(group 2)and three successive years(group 3).The biomass,MDA,CAT,POD and SOD of A.senticosus seedlings in the soil without A.senticosus(group 1)were higher than those in the soil with A.senticosus(group 2 and 3),while the protein and APX were lower than those in the soil with A.senticosus(group2 and 3).Conclusion:Plant and soil shows negative feedback regulation during the growth of A.senticosus seedlings,which reduced the activity of antioxidant enzymes.Therefore,the soil without planting A.senticosus has more advantages for the growth of A.senticosus seedlings.The results provide a basis for explaining the effect of plant-soil feedback on the growth of A.senticosus,and a theoretical basis and technical support for the technical standards of A.senticosus cultivation in farmland.