添加液去白洗涤红细胞在保存中的质量观察

The Qualitative Observation of Leukocyte-Free Red Blood Cells in Additive Solution during Storage

目的 探讨添加液去白洗涤红细胞制备的可行性及其保存期质量。方法 将去白悬浮红细胞分成6份,1份置于2~6℃冰箱中保存(对照组),其余5份用生理盐水或MAP进行洗涤,利用生理盐水、MAP及ACD中的一种来悬浮制备去白洗涤红细胞:生理盐水洗涤-生理盐水悬浮组、生理盐水洗涤-MAP悬浮组、生理盐水洗涤-ACD悬浮组、MAP洗涤-MAP悬浮组、MAP洗涤-ACD悬浮组(实验组)。各洗涤实验组间采用单因素方差分析;制备后的不同保存时间(0~35天)取样检测相关指标,将不同的分组与各时间间隔按照重复测量的析因设计进行方差分析。结果 各实验组血红蛋白含量、上清蛋白含量、白细胞残留和无菌试验结果均符合相关质量标准。K^+浓度、pH值和FHb含量在各"时间"点及"分组"内均有统计学差异,"时间*分组"间存在交互作用。结论 利用添加液可以制备保存期长的去白洗涤红细胞。

Objective To investigate the processing feasibility and quality after the storage of the leukocytes removed red blood cells in additive solution.Methods One bag of,leukocyte removed red blood cells in additive solution was divided into six equal volumes.One of them was stored in refrigerator at 4±2℃,without washing as control group whereas the others were washed by saline or MAP,suspended in saline,MAP or ACD.Saline washed-saline suspended group,saline washed-MAP suspended group,saline washed-ACD suspended group,MAP washed-MAP suspended group,and MAP washed-ACD suspended group were taken for experiments.Samples were subjected to different preservation time(0~35 d)after preparation.The variance analysis was conducted according to the factorial design of repeated measurement of different groups and time intervals.Results HGB content,supernatant protein content,residue leukocytes and sterility test were all in line with the national standards.The K^+ concentration,pH value and FHb content were statistically different at each“time”and“group”.Conclusion The leukocyte-removed erythrocytes can be washed and suspended in added liquid for a long time with viability.The suitable washed RBCs products are recommended in clinical transfusion.The washed red cells suspended in saline within 24 hours or in additive solution stored within three days would be appropriate.