肿瘤浸润炎症细胞在鼻咽癌中分布及预后相关性分析

Correlation analysis of the distribution and prognosis of tumor infiltrating inflammatory cells in nasopharyngeal carcinoma

目的:分析肿瘤浸润炎症细胞在鼻咽癌中分布及预后相关性。方法:选取2009年10月至2013年10月间在本院进行治疗鼻咽癌患者石蜡标本158例,免疫组化法分析患者机体内FOXP3、CD8+、CD4+、NE+、CD56+、CD20+、CD83+、CD1a+及tryptase+细胞分布、含量和患者预后相关性状况。结果:依据炎症细胞密度将其分成高密度组与低密度组,CD3+、CD20+、CD83+及tryptase+浸润密度和患者不同病理分类相关(P<0.05),FOXP3+、NE+及tryptase+浸润密度和患者不同N分级相关(P<0.05),FOXP3+、NE+浸润密度和患者不同临床分期相关(P<0.05);CD8+、tryptase+、NE+及FOXP3+低密度比高密度生存率高,CD1a+、CD56+高密度比低密度生存率高,差异均有统计学意义(P<0.05)。结论:肿瘤浸润肥大细胞、细胞毒性T细胞及调节性T细胞含量与鼻咽癌患者不良预后有紧密联系,CD56+NK细胞与CD1a+未成熟树突状细胞和患者良好预后有联系。

Objective:To analyze the distribution and prognosis of tumor infiltrating inflammatory cells in nasopharyngeal carcinoma.Methods:From October 2009 to October 2013 paraffin specimens of 158 patients with nasopharyngeal carcinoma in our hospital were analyzed by immunohistochemistry to detect FOXP3,CD8+,CD4+,NE+,CD56+,CD20+,CD83+,CD1 a+and tryptase+cell distribution,content and prognosis condition.Results:According to the density of inflammatory cells into the high density group and low density group,CD3+,CD20+,CD83+and tryptase+infiltration density was correlated with different pathological classification,FOXP3,NE+and tryptase+infiltration density was correlated with different N grade(P<0.05),FOXP3,NE+infiltration density was correlated with different clinical stages(P<0.05).The survival rate of CD8+,tryptase,NE+and FOXP3+was higher than that of high density group,and the survival rate of CD1 a+and CD56+was higher than that of low density,the difference was statistically significant(P<0.05).Conclusion:The content of tumor infiltrating mast cells,cytotoxic T cells and regulatory T cells is closely related to the poor prognosis of nasopharyngeal carcinoma patients.CD56+NK cells are associated with the prognosis of CD1 a+immature dendritic cells and patients.