基因重组发光菌应用于环境样品毒性的测试

The Application of Gene Recombinant Luminescent Bacteria to Environmental Sample Toxicity Test

选取基因重组发光菌E.coli HB101 pUCD607和费氏弧菌为试验生物,采用急性毒性微孔板法,发光抑制率为测试指标,分别用锌离子、汞离子作为阳性对照,研究8种重金属分别对基因重组发光菌和费氏弧菌产生的生物毒性,结果显示:锌离子毒性小、稳定性高、变异系数小,更适合作为阳性对照.利用基因重组发光菌比较了离子液体和有机溶剂的生物毒性效应,同等条件下的毒性测试结果表明:基因重组发光菌半数效应质量浓度(EC50)比费氏弧菌高,灵敏度更高;在铜离子、锰离子、铁离子和镉离子的毒性作用下,作为受试生物,基因重组发光菌的灵敏度显著高于费氏弧菌(EC50之间存在显著性差异);离子液体对基因重组发光菌的毒性显著高于有机溶剂.综上所述,基因重组发光菌应用于环境样品的毒性检测可行性较高.

Gene recombinant luminescent bacteria(E.coli HB101 pUCD607)and Vibrio fischeri were used as the ex-perimental organisms in toxicity test,and luminescence inhibition rate was used as the endpoint.The toxic micro-pore-plate method,in which Zn^2+replaced Hg^2+as positive control,was used to study the effects of eight heavy metals on toxicity tests of recombinant luminescent bacteria and Vibrio fischeri.The results showed that Zn^2+was of high stability and had the advantages of being soluble,stable and less harmful to people and the environment.There-fore,Zn^2+could replace the traditional Hg^2+as the positive reference.The toxicity test results(EC50)of the recombi-nant bioluminescent bacteria were more sensitive than Vibrio fischeri.Under the toxic effects of copper ions,manga-nese ions,iron ions and cadmium ions,the sensitivity of the recombinant bioluminescent bacteria was significantly higher than that of Vibrio fischeri.The toxicity of ionic liquids to luminescent bacteria was significantly higher than that of organic solvents.So,it is feasible to apply recombinant luminescent bacteria to the toxicity test of chemicals.