淮海地区不同年龄人群糖化血清白蛋白参考区间的建立

Establishment of GA reference intervals in Huaihai

目的初步建立淮海地区不同年龄人群糖化血清白蛋白(GA)参考区间,并对其进行临床诊断评价。方法选取2017年1月—2018年1月徐州市中心医院体检者1784名,按年龄分为5组:儿童组(0~6岁)429人、少年组(7~17岁)199人、青年组(18~40岁)203人、中年组(41~65岁)618人、老年组(≥66岁)315人。采用酶法检测其GA,分别计算95%可信区间;另选取2018年5—6月表面健康者221名,检测其GA并计算R值,进行参考区间的验证;收集2018年6月1—5日徐州市中心医院行GA检测的血清标本1367例,其中糖尿病患者血清标本149例、非糖尿病患者血清标本1218例,对建立的参考区间进行临床诊断评价。结果建立的淮海地区不同年龄人群GA参考区间分别为:儿童组6.28%~13.54%,少年组7.25%~14.93%,青年组8.32%~14.66%,中年组9.52%~15.64%,老年组10.09%~16.29%。新建立的GA参考区间诊断糖尿病的特异性为95.00%,敏感性为98.65%,误诊率为6.89%,漏诊率为1.34%。结论淮海地区糖耐量正常人群GA的参考区间为7.61%~15.37%,且随着年龄增长GA水平呈上升趋势,建议实验室设置不同年龄人群的GA参考区间。GA水平对糖尿病有诊断价值。

Objective To establish the reference intervals of glycated albumin(GA)in different age groups in Huaihai,which is evaluated by laboratory diagnosis.Methods A total of 1784 healthy subjects in the Central Hospital of Xuzhou City from January 2017 to January 2018 were enrolled.All the subjects were classified according to different ages.There were 429 children(0-6 years old),199 juniors(7-17 years old),203 young people(18-40 years old),618 middle-aged people(41-65 years old)and 315 elder people(≥66 years old).The GA levels were determined by enzymatic method.The 95%confidence intervals of reference intervals were calculated.Totally,221 healthy subjects'samples were collected in the Central Hospital of Xuzhou City from May 2018 to June 2018.The results of GA were collected,and the value of R was calculated.The reference interval was verified.There were 1367 samples which were collected from whom came to the Central Hospital of Xuzhou City from June 1th 2018 to June 5th 2018,which were contained 149 diabetes mellitus patients and 1218 non-diabetes mellitus patients.The reference interval was evaluated by laboratory diagnosis.Results The reference interval of child group was 6.28%-13.54%,that of junior group was 7.25%-14.93%,that of young group was 8.32%-14.66%,that of middle-aged group was 9.52%-15.64%,and that of elder group was 10.09%-16.29%.The specificity of the reference intervals of GA was 95.00%,the sensitivity was 98.65%,the misdiagnosis rate was 6.89%,and the missed diagnosis rate was 1.34%.Conclusions The reference interval of GA in Huaihai is 7.61%-15.37%.The level of GA increases with ages.It is recommended to set GA reference interval according to different ages.GA has a diagnostic value for diabetes mellitus.