妊娠梅毒患者外周血T/B/NK淋巴细胞亚群检测及分析

The expression of lymphocyte subgroup in peripheral blood of pregnant syphilis patients and the analysis of immune function

目的:检测妊娠梅毒患者外周血中淋巴细胞亚群及NK细胞的表达情况,探讨其在妊娠梅毒发病机制中的作用。方法:采用流式细胞仪对12例未经驱梅治疗的妊娠梅毒患者及16例血清固定的妊娠梅毒患者外周血CD3+、CD4+、CD8+、CD4+/CD8+、CD19+、NK细胞进行检测,并与12例梅毒非妊娠女性、32例健康孕妇、25例健康非妊娠女性进行比较。结果:与其他4组相比,血清固定组CD3+、CD4+、CD4+/CD8+细胞比例均显著降低(P<0.05),CD8+细胞比例显著升高(P<0.05);未治疗组CD4+细胞比例低于健康孕妇组(P<0.05),CD8+细胞比例高于梅毒非孕妇组、健康孕妇组和健康非孕妇组(P<0.05);未治疗组和血清固定组CD19+细胞比例均高于梅毒非孕妇组、健康孕妇组和健康非孕妇组(P<0.05);血清固定组NK细胞比例高于未治疗组(P<0.05);梅毒非孕妇组、健康孕妇组和正常非孕妇组各项淋巴细胞亚群水平无统计学差异(P>0.05)。结论:妊娠梅毒患者存在细胞免疫抑制,体液免疫受到影响;血清固定组出现免疫失衡,NK细胞水平下降,引起机体对梅毒螺旋体的清除能力下降。

Objective:To determine the expression of peripheral lymphocyte subgroup and nk cells in peripheral blood of syphilis patients and to investigate its clinical significance.Methods:The levels of peripheral blood lymphocyte CD3+,CD4+,CD8+,CD4+/CD8+,CD19+,NK cell were detected with flow cytometry in 12 cases of untreated pregnant syphilitic patient,16 cases of pregnant patients with syphilis sero-fixation,12 cases of non-pregnant women with syphilis,25 cases of normal controls and 32 cases of normal pregnant women was analzed.Results:The level of CD3+、CD4+、CD4+/CD8+in syphilis sero-fixation group was lower than that of the other four groups(P<0.05),the level of CD8+was higher significantly(P<0.05).The level of CD4+was lower than normal pregnant women group(P<0.05),the level of CD8+was higher than non-pregnant women with syphilis,normal pregnant women group and normal non-pregnantwomen group(P<0.05).The level of CD19+in untreated group and sero-fixation group was higher than the non-pregnant women with syphilis,normal pregnant women group and normal non-pregnant women group(P<0.05).The level of NK cell in the sero-fixation group was higher than the untreated group(P<0.05).All indicators in the three control groups had no significant difference(P>0.05).Conclusion:The prominent suppression of cellular immunity and effects of humoral immune in pregnant syphilis patients is observed.There is an imbalance of cellular immunity and the lower level of NK cell in syphilis sero-fixation group,which cause the decline of ability of removing Treponema pallidum.