维生素B6和丰诺安对X射线辐照小鼠外周血细胞的保护作用及意义

Protective effects of VB6 and fenuoan on peripheral blood cells of X-ray irradiated mice and their significance

目的观察维生素B6和丰诺安(20AA复方氨基酸)对X射线辐照小鼠外周血细胞的保护作用。方法将小鼠随机分为对照组、模型组、维生素B6组、丰诺安组和联合组。除对照组外,其余4组小鼠均接受X线照射。从照射前4 d开始至照射后第10天,对照组和模型组腹腔注射等渗盐水,维生素B6组、丰诺安组和联合组分别腹腔注射维生素B6、丰诺安、以及维生素B6和丰诺安的混合液,照射后第11天处死小鼠,检测小鼠血常规和外周血淋巴细胞亚群。结果与对照组相比,X线照射小鼠外周血白细胞总数、中性粒细胞、淋巴细胞、单核细胞、红细胞、血小板、自然杀伤(natural killer cell,NK)细胞、总T细胞、CD4^+和CD8^+T细胞数量和血红蛋白浓度均显著降低(P<0.05);CD4/CD8比值升高(P<0.05);丰诺安、维生素B6和混合液均可显著提高X线照射小鼠的白细胞总数、淋巴细胞、血小板、NK细胞、B细胞、总T细胞、CD4^+和CD8^+T细胞数量(P<0.05);混合液在提高总T细胞数量[(41.94%±10.90%)比(32.14%±5.86%)]和CD4^+T细胞数量[(33.11%±10.00%)比(25.01%±4.64%)]效果上优于维生素B6(P<0.05),在提高白细胞总数[(6.3±0.6)×10^9/L比(3.7±0.5)×10^9/L]和淋巴细胞数量[(5.3±0.4)×10^9/L比(3.4±0.3)×10^9/L]效果上优于丰诺安组(P<0.05)。结论维生素B6和丰诺安对X射线辐照小鼠外周血细胞具有保护作用,维生素B6和丰诺安联合应用优于单一应用。

Objective To observe the protective effect of vitamin B6(VB6)and Fengnuoan(20AA compound amino acid)on peripheral blood cells of X-ray irradiated mice.Methods The mice were randomly divided into five groups:control group,model group,VB6 group,Fengnuoan group and combined group.Except the control group,the mice in the other four groups received X-ray irradiation.From the 4th day before irradiation to the 10th day after irradiation,the mice in control group and the model group were injected with normal saline intraperitoneally,the mice in VB6 group,Fengnuoan group and combined group were injected intraperitoneally with VB6,fenoan,and the compound solution of VB6 and fenoan respectively.The mice were killed on the 11 th day after irradiation,and the blood routine and peripheral blood lymphocyte subsets were detected.Results Compared with the control group,the total number of leukocytes,neutrophils,lymphocytes,monocytes,erythrocytes,platelets,NK cells,total T cells,CD4^+and CD8^+T cells and hemoglobin concentration in the peripheral blood of X-ray irradiated mice were significantly reduced(P<0.05),the ratio of CD4 to CD8 was significantly increased.Fengnuoan,VB6 and compound of Fengnuoan and VB6 could increase the total number of leukocytes,lymphocytes,platelets,NK cells,total T cells,B cells,CD4^+and CD8^+T cells of X-ray irradiated mice(P<0.05).Compound was better than VB6 in increasing the number of total T cell[(41.94%±10.90%)vs(32.14%±5.86%)]and CD4^+T cell[(33.11%±10.00%)vs(25.01%±4.64%)],and better than Fengnuoan in increasing the total number of leukocytes[(6.3±0.6)×10^9/L vs(3.7±0.5)×10^9/L]and lymphocytes[(5.3±0.4)×10^9/L vs(3.4±0.3)×10^9/L].Conclusion Fengnuoan and VB6 could protect the peripheral blood cells of mice irradiated by X-ray.The protective effect of combined VB6 and fenuoan is better than that of single application.