石墨烯溶胶对逆境下欧洲山杨组培苗生长的影响

Effects of Graphene Sol on the Growth of Tissue Culture Seedlingsof Populus tremula under stress

石墨烯(Graphene)具有独特结构及优异性质,在众多领域得到广泛应用。为研究其对植物抗逆性的影响,本试验以欧洲山杨(Populus tremula)组培苗为材料,研究了NaCl和PEG-6000胁迫下石墨烯溶胶对其生长及生理特征的影响。同时还研究了两种浓度(4、7.5 mg·L^-1)石墨烯溶胶对NaCl处理培养基电导率的影响。结果表明:本试验中加入7.5 mg·L^-1石墨烯溶胶,可以提高欧洲山杨组培苗在75 mmol·L^-1 NaCl胁迫下的成活率与生根率,但所生根较细弱短小。而4 mg·L^-1石墨烯溶胶的加入也能提升苗木地上部分质量。在本试验中(75 mmol·L^-1 NaCl+4、7.5 mg·L^-1石墨烯溶胶)处理,叶片中三种保护酶(SOD、POD、CAT)活性均较单独NaCl处理有明显下降。10%PEG-6000胁迫下添加4 mg·L^-1石墨烯溶胶并不能提高植株的存活率和各生长指标。(NaCl+7.5 mg·L^-1石墨烯溶胶)处理培养基电导率低于(NaCl+4 mg·L^-1石墨烯溶胶)处理,但两者均高于NaCl单独胁迫处理的培养基电导率。研究发现,(4、7.5 mg·L^-1)石墨烯溶胶能够有效缓解NaCl胁迫对欧洲山杨组培苗的伤害,其作用机理主要与石墨烯溶胶对有机质的吸附有关。而4 mg·L^-1石墨烯溶胶对10%PEG-6000胁迫没有有益影响,石墨烯溶胶并不能缓解干旱对植株的胁迫作用。

Graphene has been widely used in many fields due to its unique structure and excellent properties.In order to study its effects on plant stress resistance,the effects of grapheme sol on growth and physiological characteristics of Populus tremula Linn under NaCl and PEG-6000 stress were studied.At the same time,the effects of graphene at two concentrations(4,7.5 mg·L^-1)on the conductivity of NaCl stress treated medium were also studied.The results showed that the survival rate and rooting rate of(Populus tremula)tissue-cultured seedlings under 75 mmol·L^-1 NaCl stress could be improved by adding 7.5 mg L^-1,but the rooting rate was weak and short.The addition of 4mg L^-1 graphene can also improve the quality of aboveground seedlings.The activities of three protective enzymes(SOD,POD and CAT)in leaves treated with(75 mmol·L^-1 NaCl+4 and 7.5 mg·L^-1 graphene)were significantly lower than those treated with NaCl alone.Adding 4 mg·L^-1 graphene under 10%PEG-6000 stress did not increase plant survival rate and growth index.(NaCl+7.5 mg·L^-1 graphene)treatment had lower conductivity than(NaCl+4 mg·L^-1 graphene)treatment,but both of them were higher than those of only NaCl stress treatment.It was found that(4,7.5 mg L^-1)graphene could effectively alleviate NaCl stress on tissue culture seedlings of Populus tremula Linn and its mechanism was mainly related to the adsorption of organic matter by graphene.However,4 mg L^-1 graphene had no beneficial effect on 10%PEG-6000 stress,and graphene could not alleviate the stress of drought on plants.