鞘氨醇单胞菌中威兰胶合成关键基因welE和welC的生物信息学分析

Bioinformatics analysis of key genes welE and welC involved in welan gum biosynthesis in Sphingomonas sp.WG

为探究威兰胶合成途径中关键基因welE和welC的结构特性及功能,运用生物信息学手段分析了welE和welC基因的序列信息,预测了其编码蛋白的理化性质、跨膜区、信号肽、磷酸化修饰、结构域及高级结构等.研究结果表明,WelE蛋白是由234个氨基酸组成的稳定亲水性蛋白质,无跨膜结构和信号肽,存在20个磷酸化位点,含有一个BY-kinase结构域,说明WelE可能参与威兰胶的生产与转运,WelE二级结构主要由α-螺旋和无规则卷曲组成,三级结构呈“圆环状”;WelC蛋白是由449个氨基酸组成的不稳定亲水性跨膜蛋白质,含有2个跨膜螺旋、无信号肽,存在39个磷酸化位点,含有一个Wzz结构域,这表明WelC可能与威兰胶链长调节有关,WelC二级结构以α-螺旋和无规则卷曲为主,三级结构呈“条状”,这些结果为解析welE和welC基因的功能以及建立威兰胶代谢调控方法奠定了理论基础.

In order to explore the structures and functions of key genes welE and welC involved in the synthesis pathway of welan gum,a series of bioinformatics methods were used in this research to analyze the sequence characteristics of welE and welC,and predict the physicochemical properties,transmembrane regions,signal peptides,phosphorylation modification,domains and spatial structures of their encoded WelE and WelC proteins.The results showed that the WelE is a stable hydrophilic protein.It consists of 234 amino acids and owns a structure without transmembrane structure and signal peptide.There are 20 phosphorylation sites and a BY-kinase domain in WelE.The structure indicates that the WelE may be involved in the production and transport of welan gum.Its secondary structure is mainly composed of α-helix and random coil,and the tertiary structure is annular.The WelC is an unstable hydrophilic transmembrane protein consisting of 449 amino acids.This protein contains two transmembrane helices,but no signal peptide.There are 39 phosphorylation sites in WelC and it contains a Wzz domain.The domain shows that the WelC may participate in the chain-length regulation of welan gum.The secondary structure is mainly composed of α-helix and random coil,and the tertiary structure is strip.These results will lay a theoretical foundation for revealing the detailed functions of welE and welC genes and further establishing new strategies for metabolic pathway regulation of welan gum biosynthesis.