基于网络药理学方法研究丹参-葛根配伍治疗冠心病的作用机制

Study on the mechanism of Danshen-Gegen in treating coronary heart disease based on network pharmacology

为探讨丹参-葛根治疗冠心病的作用机制。利用网络药理学,首先在中药系统药理学分析平台获取丹参-葛根活性成分和作用靶点,然后在TTD、DrugBank和Disgenet数据库获取冠心病作用靶点,最后利用网络药理学分析软件及相关数据库进行靶蛋白相互作用网络(PPI)构建、靶点富集分析和活性成分-靶点-通路网络构建。结果显示筛选出61个活性成分和68个共有靶点。GO分析结果显示丹参-葛根参与RNA聚合酶II启动子的转录和炎症等多种生物学过程,靶标主要集中于细胞外空间、质膜和核等部位。KEGG分析结果显示丹参-葛根配伍调节包括HIF-10、PI3K-Akt、TNF和Jak-STAT等信号通路。综上所述,丹参-葛根中的活性成分丹参酮ⅡA、木犀草素和葛根素等通过HIF-10、PI3K-Akt、TNF和Jak-STAT等信号通路对TNF、VEGFA、TP53和IL6等相关靶点发挥血压调节、血管生成和炎症反应等作用。本研究结果为进一步研究丹参-葛根的药理作用提供了参考。

To explore the mechanism of action of Danshen-Gegen on coronary heart disease.Using network pharmacology,the first system in Chinese medicine pharmacological analysis platform for Danshen-Gegen active ingredients and targets,and then in the TDD,DrugBank and Disgenet database for coronary heart disease targets,the use of network pharmacology analysis software and related database for target protein interaction network(PPI),enrichment of target analysis and active ingredients-targets-pathway network construction.The results showed that 61 active ingredients and 68 mutual targets were screened from Danshen-Gegen.GO analysis showed that Danshen-Gegen root was involved in the transcription and inflammation of RNA polymerase II promoter and other biological processes,and targets were mainly concentrated in the extracellular space.The results of KEGG analysis showed that the regulation of Danshen-Gegen included signaling pathways such as HIF-10,PI3K-Akt,TNF and Jak-STAT.Above all,some active ingredients of Danshen-Gegen,such as tanshinoneⅡA,mignonette and puerarin,play a role of regulate blood pressure,angiogenesis and inflammation by affecting HIF-10,PI3K-Akt,TNF and Jak-STAT signal pathway.These pathways are primarily associated with the common targets of TNF,VEGFA,TP53 and IL6.Results of this study provide a reference for further study of the pharmacological effects of Danshen-Gegen.