离子色谱法与国标高效液相色谱法对婴幼儿配方乳粉中糖类的测定分析

Comparative Analysis of Sugars in Infant Formula Milk Powder by Ion Chromatography and High Performance Liquid Chromatography Specified in Chinese National Standard

采用高效阴离子交换分离-脉冲安培检测法建立婴幼儿配方乳粉中葡萄糖、蔗糖、乳糖、低聚半乳糖三糖、蔗果三糖、低聚半乳糖五糖、蔗果四糖及蔗果五糖的分析方法,对4种品牌乳粉中的糖类进行定性和定量分析。结果表明:离子色谱法测定结果的相对标准偏差小于4%(n=5),加标回收率为78.0%~96.8%,检出限为3.0~12.0 mg/100 g;采用国标中高效液相色谱法与离子色谱法进行比较,2种方法对乳粉中蔗糖、乳糖、蔗果三糖的检出结果基本相同,但其他5种糖高效液相色谱法均未检出,而离子色谱法均检出相应含量,离子色谱法比高效液相色谱法灵敏度高,可同时检测婴幼儿配方乳粉中多种糖类。

A high-performance anion exchange chromatography with pulsed amperometric detection(HPAEC-PAD)was established to analyze glucose,sucrose,lactose,galactooligotrisaccharide,1-kestose,galactooligopentosaccharide,nystose and 1F-fructofuranosyl nystose in four commercial brands of infant formula.The spiked recoveries of HPAEC-PAD ranged from 78.0%to 96.8%with relative standard deviation(RSD)of 4%(n=5).Its limits of detection were between 3.0 and 12.0 mg/100 g.When HPAEC-PAD and the high-performance liquid chromatography(HPLC)specified in the Chinese national standard were applied to detect sucrose,lactose and 1-kestose in real samples,consistent results were obtained.However,the five other sugars were detected by HPAEC-PAD but not by HPLC.Accordingly,compared with HPLC,HPAEC-PAD was more sensitive and useful for the simultaneous determination of multiple sugars in infant formula.