过敏性紫癜患者B淋巴细胞Cosmc、T-合酶mRNA的表达与血清低半乳糖基化IgA1水平检测

Cosmc and T-synthase mRNA expression in peripheral B lymphocytes and serum levels of galactose-deficient IgA1 in patients with Henoch-Schonlein purpura

目的探讨外周血B淋巴细胞Cosmc、T-合酶及血清低半乳糖基化IgA1(Gd-IgA1)在过敏性紫癜(HSP)患者中的变化。方法2014年1-8月在河北医科大学第二医院皮肤性病科收集门诊或住院HSP患者56例,分为4组,即皮肤型组(22例)、关节型组(9例)、腹型组(12例)、肾型组(13例)。20例健康志愿者为健康对照组。采用实时荧光定量PCR检测患者外周血B淋巴细胞Cosmc、T-合酶mRNA表达量,凝集素亲和ELISA法检测血清Gd-IgA1水平。多组间比较采用单因素方差分析或Kruskal-Wallis H检验,两两多重比较采用LSD-t检验或Nemenyi检验,相关性分析采用Spearman秩相关检验。结果皮肤型组发病时长为(6.27±3.09)d,关节型组(5.56±3.05)d,腹型组(6.75±3.75)d,肾型组(26.23±14.12)d,差异有统计学意义(χ2=26.19,P<0.05);肾型组发病时长明显长于其他3组病例组(均P<0.05)。皮肤型、关节型、腹型、肾型组和健康对照组Cosmc mRNA相对表达量分别为0.849±0.239、0.767±0.181、0.719±0.183、0.459±0.121、1.146±0.232,各组间差异有统计学意义(F=23.37,P<0.05),各病例组均低于健康对照组(P<0.01),且肾型组低于其他3组病例组(均P<0.01)。各病例组与健康对照组的外周血B淋巴细胞T-合酶mRNA表达水平差异无统计学意义(F=1.05,P>0.05)。皮肤型、关节型、腹型、肾型紫癜组及健康对照组间血清Gd-IgA1水平差异有统计学意义(F=7.06,P<0.05),各病例组Gd-IgA1水平均高于健康对照组(均P<0.05),肾型组高于其他3组病例组(均P<0.05)。HSP组患者血清Gd-IgA1水平与Cosmc mRNA相对表达量显著负相关,rs=-0.50,P<0.01。结论HSP患者Cosmc mRNA表达水平下降,血清Gd-IgA1水平升高,二者呈负相关。

Objective To investigate changes in the expression of Cosmc and T-synthase in peripheral B lymphocytes and in serum levels of galactose-deficient IgA1(Gd-IgA1)in patients with Henoch-Sch?nlein purpura(HSP).Methods From January to August 2014,56 patients with HSP were collected from outpatient or inpatient department of dermatology and venereology in the Second Hospital of Hebei Medical University,and were divided into 4 groups,including skin type group(22 cases),joint type group(9 cases),abdominal type group(12 cases)and renal type group(13 cases).Twenty healthy volunteers served as healthy controls.Real-time fluorescence-based quantitative PCR was performed to determine the mRNA expression of Cosmc and T-synthase in peripheral B lymphocytes,and a lectin-based enzyme-linked immunosorbent assay(ELISA)to detect the serum level of Gd-IgA1.Comparisons among multiple groups were performed using one-way analysis of variance or Kruskal-Wallis H test,multiple comparisons were performed using least significant difference(LSD)-t test or Nemenyi test,and correlation analysis was performed using Spearman rank correlation analysis.Results There was a significant difference in the duration from disease onset to the clinic visit(χ2=26.19,P<0.05)among the skin type group(6.27±3.09 d),joint type group(5.56±3.05 d),abdominal type group(6.75±3.75 d),and renal type group(26.23±14.12 d),and the duration from disease onset to the clinic visit was significantly longer in the renal type group than in the other 3 groups(all P<0.05).The Cosmc mRNA expression significantly differed among the skin type group,joint type group,abdominal type group,renal type group and healthy control group(0.849±0.239,0.767±0.181,0.719±0.183,0.459±0.121,1.146±0.232,F=23.37,P<0.05),was significantly lower in the 4 patient groups than in the healthy control group(P<0.01),and lower in the renal type group than in the other 3 patient groups(all P<0.01).There was no significant difference in the T-synthase mRNA expression in peripheral B lymphocytes among the patient groups and healthy control group(F=1.05,P>0.05).The serum level of Gd-IgA1 significantly differed among the skin type group,joint type group,abdominal type group,renal type group and healthy control group(F=7.06,P<0.05).Moreover,the Gd-IgA1 level was significantly higher in the patient groups than in the healthy control group(all P<0.05),and higher in the renal type group than in the other 3 patient groups(all P<0.05).The serum level of Gd-IgA1 in the HSP patients was significantly and negatively correlated with the mRNA expression of Cosmc(rs=-0.50,P<0.01).Conclusion Decreased mRNA expression of Cosmc and increased serum levels of Gd-IgA1 were observed in patients with HSP,and there was a negative correlation between the two indices.