术前口服富氢水减轻大鼠移植肾缺血再灌注损伤的作用

Pretreatment of oral hydrogen-rich water attenuates renal ischemia-reperfusion injury of kidney transplantation in rats

目的评价口服富氢水减轻肾移植大鼠移植肾缺血再灌注损伤的作用及其机制。方法健康雄性SD大鼠24只,采用随机数字表法分为4组(n=6),术前7 d开始分别采用富氢水和普通水喂养供鼠和受鼠,建立肾移植模型。(1)对照组:供、受鼠采用普通水喂养;(2)受鼠组:受鼠采用富氢水喂养,供鼠采用普通水喂养;(3)供鼠组:受鼠采用普通水喂养,供鼠采用富氢水喂养;(4)实验组:供、受鼠均采用富氢水喂养。移植后24 h,采集受鼠下腔静脉血及移植肾,检测肾功能、氧化应激、炎症反应、肾小管上皮细胞损伤和凋亡等相关指标评价肾损伤情况,同时检测凋亡相关蛋白表达和肾组织内p38磷酸化探讨相应机制。结果与对照组相比,受鼠组、供鼠组及实验组均可见受鼠肾功能改善,受肾氧化应激损伤减轻,炎性因子生成减少,肾小管上皮细胞损伤及凋亡缓解,凋亡相关蛋白及p38磷酸化明显受抑(P<0.05)。与受鼠组相比,上述表现在供鼠组及实验组中更为明显(P<0.05)。结论术前口服富氢水可明显减轻大鼠移植肾的缺血再灌注损伤,其机制可能与抑制p38 MAPK信号通路的激活有关。

Objective To examine the protective effect of preoperatively oral hydrogen-rich water on attenuating renal ischemia-reperfusion(I/R)injury of kidney transplantation in rats and explore the related mechanism.Methods Orthotopic kidney transplantation was performed in Sprague-Dawley rats(n=6).Pretreatment of hydrogen-rich and conventional water started at 1 week pre-transplantation.Conventional water was provided for both donors and recipients in control(C)group,hydrogen-rich water for recipients and conventional water for donors in hydrogen-water-treated recipient(HWR)group,hydrogen-rich water for donors and common water for recipients in hydrogen-water-treated donor(HWD)group,hydrogen-rich water for both donors and recipients in hydrogen-water-treated donor and recipient(HWDR)group.Renal function,oxidative stress,inflammatory and apoptotic parameters were monitored at 24h post-reperfusion.The phosphorylation of p38 in kidney graft was assayed by Western blot.Results As compared with C group,renal function of recipients became ameliorated in HWR/HWD/HWDR group.Meanwhile,oxidative stress and inflammatory response were mitigated and lessened pathological injury and apoptosis of renal tubular epithelial cells coincided with suppressing apoptosis-related protein's generation and phosphorylation of p38(P<0.05).Compared with HWR group,the above differences were more significant in HWD and HWDR groups(P<0.05).Conclusions Preoperative intake of hydrogen water attenuates renal I/R injury after kidney transplantation.And suppressing the activation of p38 MAPK may be a potential mechanism.