Apelin-13对急性心肌梗死家兔的治疗作用

Effect of Apelin-13 on the treatment of acute myocardial infarction in rabbits

目的探讨Apelin-13对急性心肌梗死(AMI)家兔的治疗作用。方法 30只雄性家兔,随机分为假手术组、AMI对照组和Apelin组,各10只。术后开始给药, Apelin组耳缘静脉注射Apelin-130.1 mg/(kg·d),连续7 d, AMI对照组及假手术组注射等量生理盐水。比较三组家兔血管内皮生长因子(VEGF)、梗死边缘区微血管计数、凋亡指数(AI)、心脏重量/体重,观察三组心肌损伤程度及胶原沉积情况。结果假手术组VEGF含量、梗死边缘区微血管计数、AI、心脏重量/体重分别为(44.94±2.76)ng/L、(7.820±1.059)个、(42.84±2.65)%、(1.90±0.12)‰,显著低于AMI对照组的(59.72±4.17)ng/L、(13.540±1.704)个、(73.97±5.03)%、(2.70±0.01)‰,差异均有统计学意义(P<0.05)。Apelin组VEGF含量、梗死边缘区微血管计数分别为(68.65±4.91)ng/L、(16.130±2.220)个,均高于AMI对照组, AI及心脏重量/体重分别为(65.73±4.42)%、(2.60±0.15)‰,均低于AMI对照组,差异均有统计学意义(P<0.05)。HE染色可见假手术组家兔心肌组织结构排列整齐, AMI对照组可见心肌组织中有广泛的炎性细胞浸润,心肌纤维断裂,排列紊乱,心肌细胞坏死。Apelin-13组可见炎性细胞浸润、心肌纤维断裂比AMI对照组减轻。结论 Apelin-13可促进AMI家兔梗死周围新生血管生成、侧支循环形成,缩小心肌梗死面积,且可有效抑制心肌细胞凋亡、减轻心肌细胞损伤、改善AMI家兔心肌重构。

Objective To discuss the therapeutic effect of apelin-13 on acute myocardial infarction(AMI) in rabbits. Methods A total of 30 male rabbits were randomly divided into sham operation group, AMI control group and Apelin group, with 10 rabbits in each group. After operation, Apelin-130.1 mg/(kg·d) was injected into the ear margin of Apelin group for 7 d. The same amount of normal saline was injected into the control group and sham operation group. The vascular endothelial growth factor(VEGF), microvessel count in infarcted margin, apoptotic index(AI), heart weight/body weight were compared in three groups of rabbits, and the degree of myocardial injury and collagen deposition of three groups were observed. Results The VEGF content, microvessel count in infarcted margin, AI and heart weight/body weight were(44.94±2.76) ng/L,(7.820±1.059) pieces,(42.84±2.65)% and(1.90±0.12)‰ in sham operation group, which were significantly lower than(59.72± 4.17) ng/L,(13.540±1.704) pieces,(73.97±5.03)% and(2.70±0.01)‰ in AMI control group, and their difference was statistically significant(P<0.05). The VEGF content and microvessel count in infarcted margin were(68.65±4.91) ng/L and(16.130±2.220) pieces in Apelin group, which were higher than those in the control group, and AI and heart weight/body weight were(65.73±4.42)% and(2.60±0.15)‰ respectively, which were lower than those in the AMI control group. Their difference was statistically significant(P<0.05). HE staining showed that the myocardial tissue structure of the sham operation group was neatly arranged;AMI control group showed extensive inflammatory cell infiltration, myocardial fiber rupture, disordered arrangement, and myocardial cell necrosis;Apelin-13 group showed inflammatory cell infiltration, and myocardial fiber rupture is reduced compared with AMI control group. Conclusion Apelin-13 can promote neovascularization and collateral circulation formation around AMI rabbit infarction, reduce the area of myocardial infarction, and can effectively inhibit myocardial cell apoptosis, reduce myocardial cell damage, and improve myocardial remodeling.