老年性痴呆患者载脂蛋白E基因多态性与淀粉样β蛋白前体16-17外显子表达的相关性及意义

The relationship between serum apolipoprotein E gene polymorphism and amyloid β protein precursor 16-17 exogenous factor expression in patients with Alzheimer's disease and its clinical significance

目的探讨老年性痴呆(AD)患者血清载脂蛋白E(ApoE)基因多态性和淀粉样β蛋白前体16-17(β-APP16-17)外显因子表达相关性及其临床意义。方法选取2018-02—2019-08在郑州市第二人民医院诊治的32例AD患者纳入研究组,随机选取同期门诊或健康体检的老年自愿者32例纳入正常组,均接受聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测和相对定量竞争RNA扩增聚合酶链反应(RT-PCR)检测,对比分析2组检测结果。结果研究组ApoE基因型频率整体高于正常组(P<0.05);ApoEε4等位基因携带者的β-APP16-17表达水平高于ε2、ε3等位基因,但差异无统计学意义(P>0.05);2组有无ApoEε4等位基因间App16-17水平无显著性差异(P>0.05)。结论ApoEε4基因是AD发生的重要危险因子,其等位基因对患者的β-APP16-17表达影响不明显,但在目前发现的基因片段中,该基因的表达可为下一步的临床诊治提供新方向。

Objective To Systematic analysis of the relationship between serum apolipoprotein E(ApoE)gene polymorphism and amyloidβprotein precursor 16-17(β-APP16-17)exogenous factor expression in patients with Alzheimer's disease(AD).Methods The main part of this study Zhengzhou Second People's Hospital 32 patients with AD who were treated in our hospital from February 2018 to August 2019 were enrolled in the study group.Elderly volunteers at random time in our hospital outpatient or health check-up 32 healthy adults were enrolled in the normal group,and all received polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)detection and relative quantification of competitive RNA amplification polymerase chain reaction(RT-PCR)detection,comparative analysis of the two groups of results.Results The results of the test 2 groups of patients were statistically analyzed.The ApoEgenotype frequency of the study group was higher than that of the normal group(P<0.05).The expression level ofβ-APP16-17 in ApoEε4 allele carriers was higher than that ofε2 andε3 alleles,but the difference was not statistical significance(P>0.05).There was no significant difference in the level ofβ-APP16-17 between the two groups(P>0.05).Conclusion ApoEε4 gene is an important risk factor for AD,and its allele has no obvious effect on the expression ofβ-APP16-17 in patients,which in the gene fragments found so far,the expression of this gene can provide a new direction for the next step of clinical diagnosis and treatment.