子宫内膜癌组织中COX-2、YKL-40、NF-κB的表达及临床意义

The Expressions and Clinical Significances of COX-2,YKL-40 and NF-κB in Endometrial Carcinoma

目的探究子宫内膜癌组织中环氧合酶-2(COX-2)、甲壳质酶蛋白40(YKL-40)、核转录因子κB(NF-κB)的表达及临床意义。方法选择2014年2月至2018年12月我院妇科收集的80例子宫内膜癌组织及同期行子宫切除的40例正常子宫内膜组织标本作为研究样本,采用免疫组化方法测定组织中COX-2、YKL-40、NF-κB蛋白表达情况,分析子宫内膜癌组织蛋白表达与患者临床参数、预后的相关性。结果子宫内膜癌组织COX-2、YKL-40、NF-κB蛋白阳性表达率显著高于正常内膜组织(P<0.05);子宫内膜癌患者COX-2水平与患者FIGO分期、淋巴结转移相关(P<0.05);YKL-40、NF-κB水平与FIGO分期、分化程度、肌层浸润、淋巴结转移相关(P<0.05);Kaplan-Meier生存曲线显示,COX-2、YKL-40、NF-κB阳性表达患者总体生存率均显著低于阴性表达患者;COX多因素分析示,在矫正其他临床资料后,YKL-40、NF-κB蛋白阳性表达是子宫内膜癌患者预后不良的独立危险因素(P<0.05)。结论子宫内膜癌组织存在COX-2、YKL-40、NF-κB等阳性表达率的升高,与肿瘤侵袭性及预后相关,可作为病情判断及预后预测的参考指标。

Objective To investigate the expressions and clinical significances of cyclooxygenase-2(COX-2),chitinase protein 40(YKL-40)and nuclear factorκB(NF-κB)in endometrial carcinoma.Methods 80 cases of endometrial carcinoma tissues collected from gynecology department of the hospital from February,2014 to December,2018 and 40 cases of normal endometrial tissue specimens collected from patients undergoing hysterectomy during the same period were selected as samples.The expressions of COX-2,YKL-40 and NF-κB proteins in these tissues were determined by immunohistochemical method.The correlations between protein expressions in endometrial carcinoma tissues and clinical parameters,prognosis were analyzed.Results The positive expression rates of COX-2,YKL-40 and NF-κB proteins in endometrial carcinoma tissues were significantly higher than those in normal endometrial tissues(P<0.05).The expression of COX-2 in patients with endometrial carcinoma was related to FIGO stage and lymph node metastasis(P<0.05).The expressions of YKL-40 and NF-κB proteins were related to FIGO stage,differentiation degree,myometrial invasion and lymph node metastasis(P<0.05).Kaplan-Meier survival curve showed that the overall survival rates of patients with positive expressions of COX-2,YKL-40 and NF-κB were significantly lower than those of patients with negative expressions.Cox multivariate analysis showed that after correction of other clinical data,positive expressions of YKL-40 and NF-κB proteins were the risk factors for poor prognosis of patients with endometrial carcinoma(P<0.05).Conclusion The positive expression rates of COX-2,YKL-40 and NF-κB are high in endometrial carcinoma tissues,which are related to tumor invasion and prognosis.They can be used as reference indexes for judgment of the clinical condition and prediction of the prognosis.