miR-144、miR-451在胃癌组织中的表达及其临床意义

The Expressions and Clinical Significances of MiR-144 and MiR-451 in Gastric Cancer Tissues

目的探究胃癌患者组织中miR-144、miR-451的表达与临床病理参数及预后的关系。方法2013年6月至2014年6月,收集115例胃癌患者的癌组织及癌旁正常组织,采用实时定量PCR检测组织中miR-144、miR-451的表达水平;分析胃癌组织中miR-144、miR-451的表达与患者临床病理参数的关系,采用Kaplan-Meier生存曲线评估miR-144、miR-451的表达与患者预后的关系,并采用Cox回归模型分析胃癌患者预后的影响因素。结果胃癌组织中miR-144相对表达量为0.214±0.069,低于癌旁正常组织的1.124±0.157,miR-451相对表达量为0.354±0.087,低于癌旁正常组织的1.084±0.108,差异均有统计学意义(均P<0.05);miR-144、miR-451的表达均与肿瘤大小、淋巴结转移、远处转移及TNM分期有关(均P<0.05)。Kaplan-Meier生存分析结果表明,与miR-144高表达组、miR-451高表达组相比,miR-144低表达组、miR-451低表达组患者术后5年总生存率较低,术后中位生存时间较短(均P<0.05)。Cox回归模型发现,远处转移、较高TNM分期、miR-144低表达、miR-451低表达是影响胃癌患者预后的危险因素(均P<0.05)。结论胃癌组织中miR-144、miR-451呈低表达,其与患者病情进展和预后有关,检测胃癌组织中miR-144、miR-451的表达可能有利于患者的病情和预后判断。

Objective To explore the relationships of miR-144/miR-451 expressions in gastric cancer tissues and clinicopathological parameters and prognosis.Methods From June,2013 to June,2014,the cancer tissues and the adjacent normal tissues were collected from 115 patients with gastric cancer,and the expression levels of miR-144 and miR-451 were detected by real-time quantitative PCR.The relationships between the expressions of miR-144 and miR-451 in gastric cancer tissues and clinicopathological parameters of patients were analyzed.Kaplan-Meier survival curve was used to evaluate the relationships between the miR-144/miR-451 expressions and the prognosis of patients.Cox regression model was used to analyze the influence factors of prognosis of patients with gastric cancer.Results The relative expression of miR-144 in gastric cancer tissues was 0.214±0.069,which was lower than 1.124±0.157 in adjacent normal tissues,while the relative expression of miR-451 was 0.354±0.087,which was lower than 1.084±0.108 in adjacent normal tissues,and the differences were both statistically significant(all P<0.05).The expressions of miR-144 and miR-451 were both correlated with tumor size,lymph node metastasis,distant metastasis and TNM stage(all P<0.05).Kaplan-Meier survival analysis showed that compared to the miR-144 high expression group and miR-451 high expression group,the 5-years overall survival rates in the miR-144 low expression group and miR-451 low expression group were lower,and the median survival time was shorter after operation(all P<0.05).Cox regression model found that distant metastasis,high TNM stage,low expression of miR-144 and low expression of miR-451 were all risk factors for prognosis of patients with gastric cancer(all P<0.05).Conclusion The expressions of miR-144 and miR-451 are low in gastric cancer tissues,which are related to the progression and prognosis of patients.Detecting the expressions of miR-144 and miR-451 in gastric cancer tissues may be helpful to the judgment of patients′condition and prognosis.