基于近场熔体静电纺丝PCR微流控芯片的构建与应用

本文介绍了一种利用近场熔体静电纺丝直写技术制作PCR(聚合酶链反应,英文全称为:Polymerase chain reaction)微流控芯片的工艺。本文先介绍了利用近场熔体静电纺丝直写技术在导电玻璃上面制作PCL纤维的过程,再介绍了PCR微流控芯片的内部结构设计,接着就介绍了PCL纤维的热处理工艺以及芯片的制作方法,最后介绍了DNA提取与应用PCR微流控芯片验证PCR实验的效果。

This paper introduces a process for making PCR(Polymerase chain reaction)microfluidic chip using near-field melt electrospinning direct writing technology.This article first introduces the process of making PCL fibers on conductive glass using near-field melt electrospinning direct writing technology,then introduces the internal structure design of PCR microfluidic chip,and then introduces the heat treatment process of PCL fibers and the chip's The production method,finally introduced the DNA extraction and application of PCR microfluidic chip to verify the effect of PCR experiments.