诃子水提物抑菌活性成分稳定性

Study on the Stability of Antibacterial Active Components by Aqueous Extract of Erminalia

以番茄晚疫病原菌为供试菌,研究了诃子水提物活性成分的稳定性,为防治番茄病害、提高产量提供一定的帮助,同时为开发新型植物源农药提供理论支持。结果表明:诃子水提物具有很好的抑菌作用,其最小抑菌浓度为3.9 mg·mL-1。诃子水提物经过酸、碱处理后其在酸性条件下抑菌圈直径较大,抑菌活性较好,经过碱处理后水提物的抑菌圈直径明显减小,抑菌活性显著下降;水提物对温度、紫外照射具有良好的稳定性;蔗糖浓度为2%时,抑菌圈直径最大,抑菌效果最好,之后随着蔗糖浓度的增高,抑菌圈直径减小,抑菌活性降低;当水提物中加入Ca2+、Mg2+、Fe3+3种金属离子时抑菌圈直径增大,抗菌能力显著提高。

In this experiment,tomato-late-blight was used as the test bacteria,the stability of the active components of water extract from terminalia was studied,in order to provide some help for the prevention and control of tomato diseases,the increase of yield and the theoretical support for the development of new plant pesticides.The results showed that,the water extract of terminalia showed good bacteriostatic activity,and its minimum bacteriostatic concentration was 3.9 mg·mL-1.The water extract of terminalia had larger diameter and better antibacterial activity under acidic conditions,the diameter of bacteriostatic zone of the aqueous extract and the bacteriostatic activity decreased obviously after alkali treatment.The aqueous extract showed good stability to temperature and ultraviolet irradiation;when the concentration of sucrose was 2%,the diameter of bacteriostatic zone was the largest and the bacteriostatic effect was the best.With the increase of sucrose concentration,the diameter of bacteriostatic zone decreased and the bacteriostatic activity decreased.When Ca2+,Mg2+,Fe3+ were added to the aqueous extract,the diameter of the antimicrobial ring increased and the antibacterial ability significantly improved.