酸化蛋清粉的体外降血脂及抗氧化作用

In vitro Hypolipidemic and Antioxidant Effects of Acidified Egg White Powder

为探究酸化蛋清粉对油酸诱导Hep G2细胞建立的体外肝细胞脂肪堆积的清除作用,将质量浓度分别为4 mg/mL、2 mg/mL、0.8mg/m L的酸化蛋清粉孵育经1.0mol/L的油酸诱导24h的Hep G2细胞后,用油红O染色观察细胞内脂质沉积情况,测定细胞内TC、TG含量及SOD活性的变化,检测ROS荧光强度,测定·DPPH自由基及·OH自由基的清除率。结果显示酸化蛋清粉浓度为2 mg/mL时具有最显著的降血脂效果,TG和TC的清除率分别为27.27%±0.02%、44.66%±0.02%;SOD活性及ROS荧光检测结果显示:随着酸化蛋清粉浓度的升高,细胞内抗氧化能力呈现上升趋势;·DPPH自由基和·OH自由基的清除能力随酸化蛋清粉浓度升高呈现出显著的随剂量依赖性的上升趋势,当酸化蛋清粉浓度18 mg/m L时,其清除率分别为88.58%±0.04%、87.82%±0.03%。结果证明酸化蛋清粉具有显著的体外降血脂及抗氧化作用。

In order to investigate the effect of acidified egg white powder on the clearance of the accumulated fat in HepG2 cells induced by oleic acid in a high-fat model, the 1.0 mol/L oleic acid-induced HepG2 cells(induction time 24 h) were incubated with acidified egg white powder(mass concentration: 4 mg/mL, 2 mg/mL and 0.8 mg/mL respectively). Lipid deposition was observed with the aid of oil red O staining. The changes in TC and TG contents and SOD activity in cells, as well as the fluorescence intensity of ROS and the scavenging rates of DPPH radical and OH radical were measured. The results showed that acidified egg white powder had the most significant hypolipidemic effect when its concentration was 2 mg/mL, with the clearance rates of TG and TC as 27.27%±0.02% and 44.66%±0.02% respectively. The results of SOD activity and ROS fluorescence detection showed that with an increase of the concentration of acidified egg white powder, the antioxidant capacity of the cells showed an upward trend(The DPPH· and OH· scavenging capacities showed a significant dose-dependent upward trend).. When the concentration of acidified egg white powder was 18 mg/m L, the clearance rates were 88.58%±0.04% and 87.82%±0.03%, respectively. The results proved that acidified egg white powder had significant in vitro hypolipidemic and antioxidant effects.