槐花醇提取物促进肝癌大鼠的细胞凋亡

Apoptosis of Sophora japonica Extraction on Hepatoma Rats

探究槐花醇提取物对肝癌大鼠细胞凋亡的干预效果及作用机制。选取50只SD大鼠,10只为正常组,其余40只建立有肝癌(分为模型组、药物对照组、低、高浓度干预组)。观察各组大鼠细胞周期分布及增殖、凋亡情况,并检测各组大鼠PTEN、p-Akt、m TOR及Bcl-2、PI3K、Akt表达。研究结果显示,高浓度组大鼠癌组织处于G1期细胞比例、凋亡率、PTEN相对表达量分别为54.72%、45.34%、0.73,均高于模型组、药物对照组、低浓度组(p<0.05);增殖率、p-Akt、m TOR、Bcl-2、PI3K、Akt相对表达量分别为13.33%、1.33、1.34、1.31、1.32、1.35,均低于模型组、药物对照组、低浓度组(p<0.05)。在槐花醇提取物的干预下,肝癌大鼠癌组织细胞增殖、凋亡能力及周期分布受到明显调控,PTEN、p-Akt、m TOR及Bcl-2、PI3K、Akt表达受到明显的调控,说明槐花醇提取物能够阻滞肝癌组织细胞周期分布,抑制肝组织细胞增殖,促进肝癌组织细胞凋亡,其能力呈现浓度依赖,为肝癌症状的临床治疗提供一定参考价值。

To investigate the interventional effect and working mechanism of Sophora japonica ethanolic extract on apoptosis of hepatoma in rats. Fifty SD rats were selected, with 10 for the normal group and 40 set up to have hepatic carcinoma(which were divided into the model group, drug control group, low-and high-dose intervention group). The cell cycle distribution, proliferation and apoptosis of rats in each group were examined, and the expressions of PTEN, p-akt, m TOR, bcl-2, PI3 K and Akt were analyzed. The results showed that the proportion of cells at G1 phase, apoptosis rate and PTEN relative expression level for the high-dose intervention group were 54.72%, 45.34% and 0.73, respectively, which were all higher than those of the model group, drug control group and low-dose intervention group(p < 0.05). The proliferation rate, the relative expression levels of p-Akt, m TOR, Bcl-2, PI3 K and Akt were 13.33%, 1.33, 1.34, 1.31, 1.32 and 1.35, respectively, which were all lower than those of the model group, drug control group and low-dose intervention group(p<0.05). The intervention with sophora japonica ethanolic extract significantly regulated the cell proliferation and apoptosis of carcinoma tissues as well as the expression of PTEN, p-Akt, m TOR and the Bcl-2, PI3 K and Akt in rats with liver cancer. These results indicate that, Sophora japonica ethanolic extract can block the cell cycle distribution, inhibit cell proliferation and promote apoptosis of carcinoma tissues in rats. Such capacities depend on the concentration of sophora japonica ethanolic extract, which provides certain reference value for clinical treatment of liver cancer symptoms.