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抗铅真菌的筛选、鉴定及其对Pb^2+的吸附特性 被引量:4

Isolation and Identification of Lead-resistant Fungus and Its Adsorption Characteristics to Pb^2+
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摘要 【目的】本文从重金属污染土壤中分离出具有耐Pb活性的菌株,为土壤重金属生物修复提供参考。【方法】采用Pb^2+浓度梯度培养法驯化筛选得到耐Pb真菌1株(命名为PbW),对其进行18S rRNA测序并构建系统发育树,在不同培养条件下研究该菌株的生长特性,设置不同的Pb^2+浓度梯度研究菌株的最大抗性水平(MRL)、去除率及吸附效率。【结果】该菌株(PbW)为茎点霉属(Phoma sp.),最适生长的环境条件分别为:温度25℃,pH 7,时间4~5 d,对Pb^2+的最大耐受浓度可达6000 mg·L^-1。该菌株在Pb^2+浓度为2000 mg·L^-1时去除和吸附效果最好,吸附率可达56.28%,去除率可达到98.42%,吸附量为61.87 mg·L^-1。【结论】菌株(PbW)对土壤中的Pb^2+具有较好的吸附和去除效果,这在Pb污染土壤的微生物修复方面具有良好的应用前景。 【Objective】This paper aimed to isolate lead-resistant strains from heavy metal contaminated soil to provide a reference for soil heavy metal bioremediation.【Method】The lead-resistant strains were isolated from heavy metal-contaminated soils,and one lead-resistant fungus(named PbW)was domesticated by Pb^2+concentration gradient culture.Its 18S rRNA was sequenced,and phylogenetic tree was constructed.The maximum resistance level(MRL),removal rate and adsorption efficiency of the strain were studied by Pb^2+concentration gradient.【Result】The strain(PbW)was Phoma sp.The optimum environmental conditions were:temperature 25℃,pH 7,time 4-5 days,the maximum tolerated concentration of Pb^2+reached 6000 mg·L^-1.The strain had the best removal and adsorption effect when the concentration of Pb2+was 2000 mg·L^-1,the adsorption rate reached 56.28%,the removal rate reached 98.42%,and the adsorption a-mount was 61.87 mg·L^-1.【Conclusion】This study showed that the strain(PbW)had a good adsorption and removal effects on Pb^2+in soil,which could have a good application prospect in microbial remediation of lead-contaminated soil.
作者 赵琪琪 李海红 安凤秋 ZHAO Qi-qi;LI Hai-hong;AN Feng-qiu(College of Environmental and Chemical Engineering,Xi'an Polytechnic University,Shaanxi Xi'an 710048,China;College of Natural Resources and Environment,Northwest A&F University,Shaanxi Yangling 712100,China)
出处 《西南农业学报》 CSCD 北大核心 2020年第2期401-407,共7页 Southwest China Journal of Agricultural Sciences
基金 陕西省科技厅工业攻关项目(2017GY-15) 陕西省教育厅科研计划项目(18JK0354)。
关键词 真菌 最大抗性水平 生长特性 去除率 吸附效率 Fungus Lead Maximum resistance level Growth characteristics Removal rate Adsorption efficiency
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