青钱柳总黄酮的提取工艺优化及体内外活性研究

Study on the Optimization of Extraction Process of Total Flavonoids from Cyclocarya Paliurus and Their in vitro and in vivo Activity

目的优化青钱柳总黄酮的提取工艺,考察该组分对肥胖模型小鼠氧化应激及糖脂代谢紊乱的干预作用。方法以α-葡萄糖苷酶抑制率及DPPH自由基清除率为评价指标,采用Box-Behnken设计-响应面法结合层次分析优化青钱柳总黄酮回流提取工艺;利用高脂饲料诱导的肥胖模型小鼠考察青钱柳总黄酮的体内活性。结果青钱柳总黄酮抑制α-葡萄糖苷酶的半数抑制浓度(IC50值)为(101.22±4.94)μg·mL^-1,清除DPPH自由基的IC50值为(23.75±1.93)μg·mL^-1;能够降低肥胖模型小鼠的体质量、肝脏指数、体脂含量;降低转氨酶、血脂、血糖水平,并具有改善脂质过氧化的作用。结论优选的提取工艺简便,所制备的青钱柳总黄酮具有抗氧化、降脂、降血糖的功效,能够改善肥胖导致的小鼠氧化应激及糖脂代谢紊乱,其活性与总黄酮的质量浓度呈正相关。

Objective To optimize the extraction process of total flavonoids from Cyclocarya paliurus,and to investigate the intervention effect of total flavonoids on oxidative stress and glycolipid metabolism disorder in obese mice model.Methods Based onα-glucosidase inhibition rate and DPPH free radical scavenging rate,Box-Behnken design-response surface method combined with the hierarchical analysis were used to optimize the extraction process of total flavonoids from Cyclocarya paliurus.The in vivo activity of total flavonoids from Cyclocarya paliurus was investigated on obese mice induced by high fat diet.Results The IC50 value of total flavonoids from Cyclocarya paliurus on theα-glucosidase inhibition was(101.22±4.94)μg·mL^-1,and the IC50 value of DPPH free radicals scavenging activity was(23.75±1.93)μg·mL^-1.Total flavonoids could reduce body mass,liver index,fat content,transaminase,blood lipid,and glucose level in obese mice,and ameliorate lipid peroxidation.Conclusion The optimized extraction process was simple and feasible.Total flavonoids had antioxidant,lipid lowering,and hypoglycemic effects.And they could improve oxidative stress and metabolism disorder of glycolipid level in obese mice.The activity was positively correlated with the mass concentration of total flavonoids.